沉默VHA-c4基因对拟南芥根长和种子萌发的影响  

Effect of the Silenced VHA-c4 Gene on Root Growth and Seed Germination of Arabidopsis thaliana

作　　者：武春燕 苏杰[2] 郭荣起 高阳[1] 李国婧[1] 王瑞刚[1] Wu Chunyan;Su Jie;Guo Rongqi;Gao Yang;Li Guojing;Wang Ruigang(Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology,Inner Mongolia Agricultural University,Hohhot,010018;Vocational and Technical College of Inner Mongolia Agricultural University,Tuyouqi,014109;Agricultural Sciences of Hulunbeier,Yakeshi,162650)

机构地区：[1]内蒙古自治区植物逆境生理与分子生物学重点实验室,内蒙古农业大学,呼和浩特010018 [2]内蒙古农业大学职业技术学院,土右旗014109 [3]呼伦贝尔市农业科学研究所,牙克石162650

出　　处：《基因组学与应用生物学》2020年第4期1785-1790,共6页Genomics and Applied Biology

基　　金：国家自然科学基金项目(30460018);内蒙古自治区科技创新团队建设计划项目(201503004)共同资助。

摘　　要：为了研究植物液泡H+-ATPase c亚基VHA-c4基因在拟南芥响应非生物胁迫中的作用,本研究构建了拟南芥VHA-c4基因沉默载体,利用农杆菌介导,将线性化的沉默载体dsDNA片段整合在拟南芥基因组上,筛选获得了能够不同程度特异沉默VHA-c4基因的7个转基因株系c4-1^c4-7,并对VHA-c4基因沉默效果最好的株系c4-2进行NaCl、ABA、6%葡萄糖胁迫处理。结果显示:在特定浓度梯度的NaCl处理后,沉默株系c4-2的主根相对伸长量和种子萌发率都被明显抑制,并且被抑制效果远远高于野生型株系。然而,在特定浓度梯度的ABA和葡糖糖处理后,沉默株系c4-2的主根相对伸长量和种子的萌发率虽然也都有一定程度的抑制,但是其被抑制效果远远低于野生型拟南芥。结果显示沉默了VHA-c4基因后,显著降低了拟南芥对NaCl的耐受性,影响了其的生长发育,然而对ABA和葡糖糖的抑制作用却表现为很不敏感,这可能是因为H^+-ATPase在盐胁迫信号通路和ABA信号通路中起着不同的(正负)调控功能。In order to elucidate the roles of vacuole H+-ATPase subunit VHA-c4 genes against abiotic stress in Arabidopsis thaliana,in this study,the plasmid of VHA-c4 gene silence was constructed,and the designed double strand DNA fragments was integrated into the genome of Arabidopsis thaliana by Agrobacterium mediation,and then seven transgenic lines c4-1-c4-7 was screened to specifically silence the coding gene of VHA-c4 subunit.It was found that under the stress of NaCl,the relative root length and the germination rate of seeds of c4-2 line were inhibited to some extent and decreased more obviously than wild strain.Under the stress of ABA or glucose,the relative root length and the germination rate of seeds of c4-2 line were inhibited to some extent;however,they did not decreased obviously as wild strain.The results showed that silenced VHA-c4 gene significantly reduced the tolerance of Arabidopsis to NaCl and affected its growth and development.However,VHA-c4 gene silenced lines showed insensitivity to ABA and glucose.This may be due to the different(positive or negative)regulatory functions of H+-ATPase in salt stress signaling pathway and ABA signaling pathway.

关 键 词：液泡ATP酶 基因干扰技术 拟南芥 脱落酸 

分 类 号：Q943.2[生物学—植物学]