β-榄香烯抑制卵巢癌细胞Cavo-3增殖的作用靶点及机制  

Target and mechanism ofβ-elemene on proliferation of ovarian cancer cell line Cavo-3

作　　者：窦磊[1] 陈洋[2] 李芳梅[1] 庞晓燕[1] 张颐[1] DOU Lei;CHEN Yang;LI Fang-mei;PANG Xiao-yan;ZHANG Yi(Department of Gynecology,the First Affiliated Hospital,China Medical University,Shenyang 110001,China;Institute of Oncology,the First Affiliated Hospital,China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院妇科,辽宁沈阳110001 [2]中国医科大学附属第一医院肿瘤研究所,辽宁沈阳110001

出　　处：《解剖科学进展》2020年第3期255-257,共3页Progress of Anatomical Sciences

基　　金：辽宁省中央引导地方科技发展专项(2019JH6/10400006);中国医科大学2019年度临床医学培育学科支持计划(妇产科学-机器人)。

摘　　要：目的研究β-榄香烯对人卵巢癌细胞Cavo-3增殖的影响及机制探索。方法体外培养卵巢癌Cavo-3细胞,用MTT实验和集落形成实验检测β-榄香烯对卵巢癌Cavo-3细胞增殖的影响,Western blot实验检测β-榄香烯对Cavo-3细胞EGFR及其下游信号通路的影响。结果β-榄香烯以浓度和时间依赖性方式抑制卵巢癌Cavo-3细胞的增殖;Western blot实验发现β-榄香烯能显著抑制EGFR及其下游通路磷酸化水平,而不影响总蛋白水平。结论β-榄香烯通过EGFR信号通路抑制卵巢癌Cavo-3细胞的增殖,揭示了β-榄香烯发挥抗卵巢癌作用的新机制。Objective To investigate the effect and mechanism ofβ-elemene on the proliferation of ovarian cancer cavo-3 cells.Methods Ovarian cancer cavo-3 cells were cultured in vitro,effect ofβ-elemene on the proliferation of ovarian cancer cavo-3 cells was determined by MTT and colony formation assay,at the same time Western blot assay was used to detect the effect ofβ-elemene on EGFR and its downstream signaling pathway in ovarian cancer cavo-3 cells.Resultsβ-elemene inhibited the proliferation of ovarian cancer cavo-3 cells in a concentration-dependent and timedependent manner,western blot analysis showed thatβ-elemene significantly inhibited the phosphorylation of EGFR and its downstream pathway,without affecting the total protein level.Conclusionβ-elemene inhibits the proliferation of ovarian cancer cavo-3 cells through the EGFR signaling pathway and plays an anti-tumor role.

关 键 词：Β-榄香烯 卵巢癌 增殖 EGFR通路 

分 类 号：R737.31[医药卫生—肿瘤]