基因沉默ILK对胰腺癌细胞Panc-1细胞增殖、凋亡与侵袭的影响  

Effect of ILK gene silencing on proliferation, apoptosis and invasion of pancreatic cancer PANC-1 cells

作　　者：朱相宇[1] 徐正馨 苏春晓 白静慧[1] ZHU Xiang-yu;XU Zheng-xin;SU Chun-xiao;BAI Jing-hui(Liaoning Cancer Hospital general medicine(VIP ward),Shenyang 110042;Respiratory specialty of integrated traditional Chinese and Western Medicine,Liaoning University of traditional Chinese Medicine,Shenyang 110034,China)

机构地区：[1]辽宁省肿瘤医院综合内科(特需病房),辽宁沈阳110042 [2]辽宁中医药大学,辽宁沈阳110034

出　　处：《解剖科学进展》2020年第3期321-324,共4页Progress of Anatomical Sciences

基　　金：辽宁省博士启动基金(20141184)。

摘　　要：目的探讨基因沉默整合素连接激酶(ILK)对胰腺癌细胞(Panc-1)细胞增殖、凋亡与侵袭的影响。方法培养胰腺癌Panc-1细胞,构建ILK-specific shRNA慢病毒载体后对Panc-1细胞进行转染,qPCR与Western Blot方法验证干扰基因片段的有效性,MTT实验检测转染后胰腺癌细胞增殖能力的变化,流式细胞仪检测转染后胰腺癌细胞周期的变化,Transwell实验检测转染后胰腺癌细胞侵袭能力的改变。结果成功构建siRNA-ILK慢病毒载体,转染组细胞的ILK mRNA及蛋白表达明显低于空病毒组及阴性对照组。基因沉默ILK的胰腺癌Panc-1细胞增殖能力显著降低,停滞在G0/G1的细胞数显著增多,G2/M期细胞数明显减少,侵袭能力显著降低(P<0.01)。结论基因沉默ILK能抑制胰腺癌细胞Panc-增殖与侵袭能力,并促进凋亡。Objective To investigate the Effect of ILK gene silencing on proliferation, apoptosis and invasion of pancreatic cancer PANC-1 cells. Methods PANC-1 cells were cultured and transfected with ILK-specific shRNA lentivirus vector. qPCR and Western blot methods were used to verify the effectiveness of the interference gene fragments. MTT assay was used to detect the proliferation ability of PANC-1 cells, flow cytometry was used to detect the cell cycle of PANC-1 cells, and transwell assay was used to detect the invasion of pancreatic cancer cells after transfection. Results SiRNA-ILK lentivirus vector was constructed successfully, and the infection efficiency of PANC-1 cells was over 80%. The expression of ILK mRNA and protein in PANC-1 cells was significantly lower than that in contral group and negative group. The proliferation ability of PANC-1 cells with ILK gene silencing was decreased significantly, the number of cells stagnating in G0/G1 was increased significantly, and the number of cells in G2/M phase was decreased significantly, invasive capacity of PANC-1 cells was decreased significantly(P<0.01). Conclusion Silencing ILK gene could inhibit the proliferation and invasion of panc-1 cells and promote apoptosis.

关 键 词：整合素连接激酶 PANC-1细胞 增殖 凋亡 侵袭 

分 类 号：R735.9[医药卫生—肿瘤]