凤尾蕨对铅污染土壤的修复机理研究  

Study on the rehabilitation mechanism of Pteris crassipes on lead contaminated soil

作　　者：徐晨茗 鲍立宁 张瑾[1,2] 姜宇 XU Chenming;BAO Lining;ZHANG Jing;JIANG Yu(School of Environment and Energy Engineering,Anhui Jianzhu University,Hefei 230601;Key Laboratory of Water Pollution Control and Wastewater Reuse in Anhui Province,Anhui Jianzhu University,Hefei 230601)

机构地区：[1]安徽建筑大学环境与能源工程学院,合肥230601 [2]安徽建筑大学水污染控制与废水资源化安徽省重点实验室,合肥230601

出　　处：《安徽农业大学学报》2020年第2期260-266,共7页Journal of Anhui Agricultural University

基　　金：安徽省自然科学基金(1708085M B50);安徽省科技重大专项(18030801106);安徽省高校自然科学研究重大项目(KJ2016SD14);安徽省重点研究与开发计划(1704a0902006);安徽省科技重大专项(17030801028)共同资助。

摘　　要：以景观植物凤尾蕨作为供试植物,研究其对土壤铅污染的修复效果,并通过分析凤尾蕨植物酶、土壤酶活性的变化,铅积累量的变化等探讨其可能的修复机理。结果表明:叶绿素、植物POD酶(过氧化物酶)、植物CAT酶(过氧化氢酶)一系列指标,在0~500 mg·L-^1时随着铅浓度的增加而上升,植物SOD酶(超氧化物歧化酶)在铅胁迫下呈下降趋势,表明凤尾蕨具有一定的耐铅性能;凤尾蕨可以吸收高浓度Pb,其地下部分与地上部分的铅积累量均随土壤铅浓度的升高而增加,地下部铅最大富集量82.578 mg·kg-^1、地上部铅最大富集量16.153 mg·kg-^1,且地下部分和地上部分的富集系数均大于1,其中地下部最大富集系数达10.819,表明凤尾蕨具有将土壤中重金属富集在植物体内的能力;在低浓度Pb(0~500mg·L-^1)处理时,蔗糖酶、脲酶、磷酸酶三种酶活性相对于对照组有所增加,表明凤尾蕨根际微生物在低浓度铅污染情况下,可协同植株强化对重金属污染土壤的修复作用。In this study, the landscape plant Pteris crassipes was used as the experimental plant to study its remediation effect on soil lead pollution, and the possible remediation mechanism was explored by analyzing the changes of plant enzymes, soil enzyme activities and lead accumulation in Pteris crassipes. The results showed that chlorophyll, plant POD enzyme and plant CAT enzyme increased with the increase of lead concentration in the range of 0-500 mg·L-^1, while plant SOD enzyme showed a downward trend under lead stress, indicating that Pteris crassipes had a certain lead tolerance.Pteridoptera crassifolia can absorb high concentration of lead. The accumulation of lead in underground and above ground parts increases with the increase of soil lead concentration. The maximum concentration of lead in underground part is 82.578 mg·kg-^1, and that in aboveground part is 16.153 mg·kg-^1. The enrichment coefficients of underground part and aboveground part are all greater than 1, and the maximum concentration in underground part is 82.578 mg·kg-^1. The coefficient is 10.819, which indicates that Pteris crassipes has the ability to enrich heavy metals in soil in plants.The activities of sucrase, urease and phosphatase increased in low concentration of Pb(0-500 mg·L-^1) treatment compared with the control group, The results showed that rhizosphere microorganisms of Pteridoptera crassifolia could cooperate with plants to strengthen the remediation of heavy metal contaminated soil under the condition of low concentration of lead pollution.

关 键 词：铅污染 植物修复 凤尾蕨 超积累植物 

分 类 号：X173[环境科学与工程—环境科学]