基于Biolog微平板技术解析典型煤矿区煤层微生物代谢及其多样性分析  

Analysis of microbial metabolism diversity of coal seam in typical coal mining areas based on biolog microplate technology

作　　者：柳炳俊 王永 金刚 LIU Bingjun;WANG Yong;JIN Gang(Coal Mining National Engineering Technology Research Institute,Huainan 232000;Anhui Kunlang New Energy Technology Co.,Ltd.,Huainan 232000)

机构地区：[1]煤炭开采国家工程技术研究院,淮南232000 [2]安徽坤朗新能源技术有限公司,淮南232000

出　　处：《安徽农业大学学报》2020年第2期275-282,共8页Journal of Anhui Agricultural University

基　　金：国家重点研发计划项目(2016YFC0801404)资助。

摘　　要：为研究不同煤矿区煤层微生物群落的代谢活性差异,找出能够激发煤层微生物活力的最佳碳源。首次采用厌氧条件下Biolog-ECO微平板技术,以我国3个典型煤矿区为研究对象(寺河矿简称SH,张北矿简称ZB,贵二矿简称GE),揭示煤层微生物代谢功能多样性特征。研究结果发现,张北矿样品第15天的平均吸光度值0.65±0.035,显著高于其他两矿,表明其微生物的代谢活力较最高;张北矿样品在第15天的多样性指数(Shannon指数、McIntosh指数、Simpson指数)也相对较高。通过主成分分析发现,煤层微生物群落在提供外源单一碳源进行代谢时,较喜欢利用L-基丙氨酸、4-羟基苯甲酸、2-羟苯甲酸、苯乙基胺含苯环的化合物。这些单一碳源或许可以作为煤层的外源营养物,加快煤层原位微生物甲烷代谢,使得生物成因煤层甲烷气体快速产出。The purpose of this paper was to study the differences in metabolic activities of coalbed microbial communities in different coal mining areas, and to find the best carbon source that can stimulate the microbial activity of coal seams. For the first time, Biolog-ECO microplate technology under anaerobic conditions was used to reveal the diversity of microbial metabolic functions in three typical coal mining areas in China(Sihe Mine abbreviated as SH, Zhangbei Mine as ZB, Guier Mine as GE). The results showed that the average well color development of Zhangbei mine samples on the 15 th day was 0.65 ±0.035, which was significantly higher than that of the other two mines, indicating that the metabolic activity of microorganisms was the highest, and the diversity index(Shannon index, McIntosh index, Simpson index) of Zhangbei mine samples was relatively high. Principal component analysis found that coalbed microbial communities prefer to use L-alanine, 4-hydroxybenzoic acid, 2-hydroxybenzoic acid, phenylethylamine when providing carbon source for metabolism. These carbon sources may serve as exogenous nutrients to accelerate methane metabolism of in situ microorganisms in coal seams, and enable the rapid production of biogenic coal-bed methane.

关 键 词：Biolog-ECO微平板技术 代谢功能多样性 生物成因煤层甲烷 

分 类 号：X172[环境科学与工程—环境科学]