Free and Hidden Fumonisins in Hordei Fructus Germinatus and Their Transfer to the Decoction  

作　　者：Ling Hu Hai-Qiang Zhu Mei-Lin Ni Jian Yang Chuan-Zhi Kang Lan-Ping Guo 

机构地区：[1]Ningbo Academy of Inspection and Quarantine,China Academy of Chinese Medical Sciences,Beijing,China [2]Ningbo,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,China

出　　处：《World Journal of Traditional Chinese Medicine》2020年第1期106-111,共6页世界中医药杂志（英文）

基　　金：the National Natural Science Foundation of China (No. 81891014,81603241);key project at central government level The ability establishment of sustainable use for valuable Chinese medicine resources (No. 2060302);the National Key Research and Development Program 250 of China (No. 2017YFC1700701);the Research Program for Basic Public Welfare of Zhejiang Province (No. LGN18H280003)。

摘　　要：Objective:This study aimed to determine the free and hidden fumonisins in Hordei Fructus Germinatus samples and to investigate the transfer rates of these mycotoxins from Hordei Fructus Germinatus to its decoction.Materials and Methods:The contamination levels of free and hidden fumonisins in a total of 60 Hordei Fructus Germinatus samples were analyzed using high-performance liquid chromatography coupled to tandem mass spectrometry(LC-MS/MS).The decoction procedure was simulated with a highly contaminated Hordei Fructus Germinatus sample,and fumonisins in the resulting decoction preparation were determined by LC-MS/MS.Results:Among all the samples,8.3%were contaminated with free fumonisins(FB1 and/or FB2)and 13.3%were contaminated with total fumonisins(free+hidden,measured as hydrolyzed fumonisins,i.e.,HFB1 and/or HFB2).The concentrations of FB1 and HFB1 reached up to 83 and 95μg/kg,respectively,whereas FB2 and HFB2 were detected only in traces.The transfer rates of free and total fumonisins from Hordei Fructus Germinatus to the decoction were 71.8%and 83.3%for FB1 and FB2,respectively.In comparison,much lower transfer rates were found for total fumonisins,i.e.,38.2%and 24.7%for HFB1 and HFB2,respectively.Conclusion:The incidence and contamination levels of free and hidden fumonisins in Hordei Fructus Germinatus samples were generally low.Regarding decoction preparation,the transfer rates of free fumonisins into the decoction were high,whereas a large part of hidden fumonisins were retained in Hordei Fructus Germinatus rather than migrating into water.

关 键 词：DECOCTION FUMONISINS hidden fumonisins Hordei Fructus Germinatus liquid chromatography coupled to tandem mass spectrometry transfer rate 

分 类 号：R283[医药卫生—中药学]