补肾调经方对PCOS模型大鼠性激素、卵巢形态及TGF-β1/smad通路的影响  

Effects of Bushen Tiaojing decoction on sex hormone,ovary morphology and TGF-β1/smad pathway in model rats with PCOS

作　　者：张伟卿 刘霞 李栗 李朝悦 张旭 唐倩 ZHANG Weiqing;LIU Xia;LI Li;LI Chaoyue;ZHANG Xu;TANG Qian(Xinle Hospital, Xinle 050700, Hebei, China;Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang 050011, Hebei, China;The People’s Hospital of Pingshan County, Pingshan 050400, Hebei, China)

机构地区：[1]河北省新乐市医院,河北新乐050700 [2]河北省中医院,河北石家庄050011 [3]河北省平山县人民医院,河北平山050400

出　　处：《现代中西医结合杂志》2020年第20期2188-2193,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省中医药管理局科研计划项目(2019473)。

摘　　要：目的探讨补肾调经方对多囊卵巢综合征(PCOS)模型大鼠性激素、卵巢形态及转化生长因子-β1(TGF-β1)/smad通路的影响。方法将60只雌性SD大鼠随机分为空白组、模型组、补肾调经方低剂量组、补肾调经方中剂量组、补肾调经方高剂量组与炔雌醇环丙孕酮组,每组10只。除空白组外,其余组大鼠采用丙酸睾酮+注射用绒促性素建立PCOS模型。造模成功后,补肾调经方低、中、高剂量组给予0.264 g/(kg·d)、0.528 g/(kg·d)、1.056 g/(kg·d)的补肾调经方溶液灌胃,炔雌醇环丙孕酮组给予临床剂量1倍的炔雌醇环丙孕酮溶液灌胃,空白组、模型组给予等体积蒸馏水灌胃,均1次/d,连续12 d。末次灌胃结束后次日,采用放射免疫法测定各组大鼠血清黄体生成激素(LH)、睾酮(T)、促卵泡成熟激素(FSH)、雌二醇(E2)水平;光镜下观察各组大鼠卵巢组织形态学变化,并计算卵泡数目;蛋白免疫印迹法检测各组大鼠卵巢组织中TGF-β1、Smad3、Smad4和Smad 7蛋白表达情况。结果与模型组比较,补肾调经方中、高剂量组与炔雌醇环丙孕酮组大鼠血清LH、T水平及卵巢组织中Smad 7蛋白表达量均明显降低,血清FSH、E2水平及卵巢组织中TGF-β1、Smad3、Smad4蛋白表达量均明显升高,卵巢质量减轻,卵泡数目减少,差异均有统计学意义(P均<0.05);与炔雌醇环丙孕酮组比较,补肾调经方高剂量组大鼠血清LH、T水平及卵巢组织中Smad 7蛋白表达量更低,血清FSH、E2水平及卵巢组织中TGF-β1、Smad3、Smad4蛋白表达量更高,卵巢质量更轻,卵泡数目更少,差异均有统计学意义(P均<0.05)。补肾调经方各剂量组与炔雌醇环丙孕酮组大鼠卵巢病理性改变均呈不同程度改善,以补肾调经方高剂量组改善最为明显。结论补肾调经方可有效调节PCOS大鼠性激素分泌,促进卵泡发育和排卵,能够改善卵巢形态,机制可能与调控TGF-β1/Smad信号通路的激活有关。Objective It is to investigate the effects of Bushen Tiaojing decoction(BSTJD)on sex hormone,ovarian morphology and transforming growth factor-β1(TGF-β1)/smad pathway in rats with polycystic ovary syndrome(PCOS).Methods Sixty female SD rats were randomly divided into 6 groups:blank group,model group,low-dose BSTJD group,medium-dose BSTJD group,high dose BSTJD group and ethinyl estradiol cyproterone group,each group had 10 rats.Except for the blank group,the rats in the other groups were given testosterone pro6pionate+chorionic gonadotropin for injection to establish PCOS models.After successful modeling,the low,medium and high dose BSTJD groups were given 0.264 g/(kg·d),0.528 g/(kg·d),1.056 g/(kg·d)BSTJD solution by gavage,the ethinylestradiol cyproterone group was given intragastric administration of ethinylestradiol cyproterone solution at twice the clinical dose,and the blank group and model group were given the same volume of distilled water by gavage,once per day for 12 consecutive days.The day after the last gavage,the levels of serum luteinizing hormone(LH),testosterone(T),follicle stimulating hormone(FSH),and estradiol(E2)in each group were measured by radioimmunoassay;the morphological changes of ovary tissues in each group were observed under light microscope and the number of follicles were counted;the expression of TGF-β1,Smad3,Smad4 and Smad 7 protein in ovary tissues of each group was detected by Western blotting.Results Compared with the model group,the levels of serum LH and T and the expression of Smad 7 protein in the ovarian tissues of the middle and high dose BSTJD groups and the ethinyl estradiol cyproterone group were significantly reduced,the levels of serum FSH and E2 and the expression of TGF-β1,Smad3,Smad4 protein in the ovarian tissues were significantly increased,ovarian quality was reduced,the number of follicles was reduced,the differences were statistically significant(all P<0.05);compared with the ethinyl estradiol cyproterone group,the levels of serum LH and T and the expre

关 键 词：多囊卵巢综合征 大鼠 补肾调经方 性激素 卵巢形态 

分 类 号：R-332[医药卫生]