恒清Ⅰ号方对血管性痴呆大鼠行为学作用及机制研究  

Study on the effect of HengqingⅠdecoction on behavior of vascular dementia rats and its mechanism

作　　者：孟胜喜[1] 霍清萍[1] 王兵[1] 付剑亮[1] 彭文波[1] 王宇新[1] 梁芳[1] 汪天湛[1] 张洪艳[1] 余忠海 彭学丰 李学敏[1] 曹健美 李文涛[3] MENG Shengxi;HUO Qingping;WANG Bing;FU Jianliang;PENG Wenbo;WANG Yuxin;LIANG Fang;WANG Tianzhan;ZHANG Hongyan;YU Zhonghai;PENG Xuefeng;LI Xuemin;CAO Jianmei;LI Wentao(The Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University,Shanghai 200233,China;Graduate School,Shanghai University of TCM,Shanghai 201203,China;Shanghai Municipal Hospital of TCM, Shanghai 200071,China)

机构地区：[1]上海交通大学附属第六人民医院,上海200233 [2]上海中医药大学研究生院,上海201203 [3]上海市中医医院,上海200071

出　　处：《现代中西医结合杂志》2020年第19期2071-2075,共5页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：上海市科委2019年度“科技创新行动计划”临床医学领域科技支撑项目(19401970600);上海市科委中医引导类项目(19401932500);上海市进一步加快中医药事业发展三年行动计划(2018-2020年);中医药重大临床研究项目[ZY(2018-2020)-CCCX-4010];上海交通大学医学院中西医结合创新基金项目(18zxy002);上海交通大学医学院2019年度教师培训发展项目(JFXM201909);上海交通大学2020年教育教学研究项目(JYJX200025)。

摘　　要：目的探讨恒清Ⅰ号方对血管性痴呆大鼠行为学的作用及其机制。方法将60只SD大鼠随机分为假手术组、模型组、恒清Ⅰ号方低剂量组、恒清Ⅰ号方中剂量组、恒清Ⅰ号方高剂量组及欧来宁组,每组10只。采用双侧颈总动脉永久性结扎法制备血管性痴呆大鼠模型。造模7 d后,恒清Ⅰ号方低、中、高剂量组分别给予0.268 g/mL、0.535 g/mL、1.070 g/mL恒清Ⅰ号方灌胃,欧来宁组给予欧来宁溶液灌胃,假手术组、模型组给予等量生理盐水灌胃,均2次/d,持续灌胃30 d。采用Morris水迷宫实验评估各组大鼠的行为学变化,然后测定各组大鼠海马组织中脑源性神经生长因子(BDNF)水平和脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、多巴胺(DA)、5-羟色胺(5-HT)、去甲肾上腺素(NE)水平。结果与模型组和恒清Ⅰ号方低剂量组比较,恒清Ⅰ号方中、高剂量组及欧来宁组大鼠的逃避潜伏期明显缩短(P均<0.05),跨越平台次数明显增多(P均<0.05);与恒清Ⅰ号方中剂量组和欧来宁组比较,恒清Ⅰ号方高剂量组逃避潜伏期明显缩短(P均<0.05),跨越平台次数明显增多(P均<0.05)。与模型组和恒清Ⅰ号方低剂量组比较,恒清Ⅰ号方中、高剂量组及欧来宁组大鼠海马组织中BDNF水平及脑组织中SOD、GSH-Px、5-HT、DA、NE水平均明显增高(P均<0.05),脑组织中MDA水平均明显降低(P均<0.05);与恒清Ⅰ号方中剂量组和欧来宁组比较,恒清Ⅰ号方高剂量组大鼠海马组织中BDNF水平及脑组织中SOD、GSH-Px、5-HT、DA、NE水平均明显增高(P均<0.05),脑组织中MDA水平均明显降低(P均<0.05)。结论恒清Ⅰ号方可能通过升高大鼠海马组织BDNF水平及脑组织SOD、GSH-Px、5-HT、DA、NE水平,降低脑组织MDA水平,从而改善血管性痴呆大鼠学习与记忆能力。恒清Ⅰ号方的量效关系呈正相关,高剂量恒清Ⅰ号方的作用优于欧来宁。Objective It is to explore the effect and mechanism of Hengqing No.1 prescription on the behavior of vascular dementia rats.Methods Sixty SD rats were randomly divided into sham operation group,model group,Hengqing No.1 prescription low-dose(HQ-L)group,Hengqing No.1 prescription middle-dose(HQ-M)group,Hengqing No.1 prescription high-dose(HQ-H)group,and Oulaining group,10 rats in each group.The rat models of vascular dementia were established by permanent ligation of bilateral common carotid arteries.After 7 days of modeling,the HQ-L group,HQ-M group and HQ-H group were respectively given 0.268 g/mL,0.535 g/mL,1.070 g/mL Hengqing No.1 prescription by gavage,and the Oulaining group was given Oulainin solution by gavage,the sham-operation group and the model group were given the same amount of normal saline,all twice a day,and continued for 30 days.Morris water maze experiment was used to evaluate the behavioral changes of rats in each group,and then the levels of brain-derived neurotrophic factor(BDNF)in the hippocampus tissue,and superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),malondialdehyde(MDA),dopamine(DA),serotonin(5-HT),norepinephrine(NE)in brain tissue of the rats in every group were detected.Results Compared with the model group and the HQ-L group,the escape latency of rats in the HQ-M group,HQ-H group and Oulaining group was significantly shortened(all P<0.05),and the number of crossing platforms was significantly increased(all P<0.05);compared with HQ-M group and Oulaining group,the escape latency of rats in the HQ-H group was significantly shortened(all P<0.05),and the number of crossing platforms was significantly increased(all P<0.05).Compared with the model group and the HQ-L group,the levels of BDNF in the hippocampus tissues and the levels of SOD,GSH-Px,5-HT,DA and NE in the brain tissue were increased significantly(all P<0.05),and the level of MDA in brain tissue was decreased significantly in the HQ-M group,HQ-H group and Oulaining group(all P<0.05);compared with HQ-M group and Oulain

关 键 词：血管性痴呆 恒清Ⅰ号方 脑源性神经生长因子 氧化应激 单胺类神经递质 

分 类 号：R-332[医药卫生]