米卡芬净中间体FR901379的分离纯化  

Separation and Purification of Micafungin Intermediate FR901379

作　　者：李敏[1] 谢新宇[1] 王晶珂[1] 郑学丽[1] 张雪霞[1] LI Min;XIE Xinyu;WANG Jingke;ZHENG Xueli;ZHANG Xuexia(Hebei Industry Microbial Metabolic Engineering&Technology Research Center,National Engineering Research Center of Microbial Medicine,New Drug Research&Development Company of NCPC,Shijiazhuang 050015)

机构地区：[1]华北制药集团新药研究开发有限责任公司,微生物药物国家工程研究中心,河北省工业微生物代谢工程技术研究中心,河北石家庄050015

出　　处：《中国医药工业杂志》2020年第6期784-788,共5页Chinese Journal of Pharmaceuticals

基　　金：河北省工业微生物代谢工程技术研究中心运行绩效后补助经费(199676154H)。

摘　　要：采用大孔树脂脱色、吸附解吸-溶剂结晶的方法开发米卡芬净中间体FR901379的分离纯化工艺。结果表明,D303筛选为最佳脱色树脂,LX-18为最佳吸附树脂。通过动态吸附和解吸的单因素影响试验优选得到树脂的最佳分离纯化条件如下:吸附流速为1.5~2 BV/h,解吸剂为80%乙醇,结晶溶剂为丙酮。此试验条件经工艺验证,所得粗粉纯度≥85%,提取收率大于70%。A separation and purification process of FR901379,an intermediate of micafungin,was developed by decolorization of macroporous resin,adsorption,desorption and solvent crystallization.The results showed that D303 was the optimal decolorizing resin and LX-18 was the optimal adsorbing resin.The separation and purification conditions of the resin were optimized by single factor experiments of dynamic adsorption and desorption.The obtained optimal parameters were as follows:the adsorption flow rate was 1.5-2 BV/h,the desorbent was 80%ethanol,and the crystallization solvent was acetone.The experimental conditions were proved to be feasible.The purity of crude powder was no less than 85%and the extraction yield was over 70%.

关 键 词：米卡芬净 中间体 FR901379 分离 纯化 大孔树脂 

分 类 号：R914.4[医药卫生—药物化学]