生长前期甘蔗叶片糖分含量及蔗糖磷酸合成酶基因表达分析  

Characterization of Sugar Content and SPS Expression Parttern in Leaves at the Early Growth Stage of Sugarcane

作　　者：陈忠良 秦翠鲜 桂意云 廖芬 汪淼 王震 龙明华[2] 黄东亮 Chen Zhongliang;Qin Cuixian;Gui Yiyun;Liao Fen;Wang Miao;Wang Zhen;Long Minghua;Huang Dongliang(Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi),Ministry of Agriculture and Rural Affairs,Guangxi Key Laboratory of Sugarcane Genetic Improvement,Sugarcane Research Istitute,Guangxi Academy of Agricultural Sciences,Nanning,530007;College of Agricul-ture,Guangxi University,Nanning,530005)

机构地区：[1]广西壮族自治区农业科学院甘蔗研究所,广西甘蔗遗传改良重点实验室,农业农村部广西甘蔗生物技术与遗传改良重点实验室,南宁530007 [2]广西大学农学院,南宁530005

出　　处：《分子植物育种》2020年第13期4229-4235,共7页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2018YFD1000500-3);广西科技重大专项(桂科AA17202012);广西自然科学基金项目(2016GXNSFBA380168,2018GXNSFA138151,2018GXNSFAA138013)共同资助。

摘　　要：本研究比较高糖(GT28,ROC22)、中糖(YT71-210)和低糖(GT86-877)基因型甘蔗生长前期叶片中的糖分含量、叶绿素含量、蔗糖代谢相关酶活性及SPS家族基因的表达水平,以探究不同甘蔗基因型生长前期的蔗糖代谢情况,为全面了解甘蔗整个生命周期的蔗糖代谢机制提供依据。结果表明,各甘蔗基因型蔗糖代谢指标与茎中糖分含量有一致性,均呈现高糖品种>中糖品种>低糖品种趋势。SofSPSA基因家族在甘蔗苗期的叶片蔗糖积累过程中mRNA水平的表达量较低,B、C、D家族基因相对表达水平较高。甘蔗的高、低糖基因型与叶片蔗糖代谢相关酶活性有着密切关系,并影响糖分积累。与低糖品种相比,高糖品种甘蔗叶片的叶绿素含量、SP家族基因的表达水平、糖分含量及蔗糖代谢相关酶活性相对较高,表明高糖品种甘蔗叶片蔗糖代谢水平更加活跃。The sugar content,chlorophyll content,activities of sucrose metabolism-related enzymes and SPS gene expression level in the leaves of sugarcanes with high sugar(GT28,ROC22),medium sugar(YT71-210)and low sugar(GT86-877)at the early stage of the growth were compared to explore the sucrose metabolism mechanism of different sugarcane genotypes.This work provided a basis for a comprehensive understanding of sucrose metabolism mechanism in sugarcane throughout its life cycle.The sucrose metabolic indexes of all sugarcane genotypes were consistent with the sugar content in stems,showing high sugar genotype>medium sugar genotype>low sugar genotype.The relative expression level of SofSPSA gene was less involved in sucrose accumulation in sugarcane leaves at seedling stage,but B,C and D family genes was higher.Sugarcane genotypes are closely related to the activities of sucrose metabolism-related enzymes in leaves and affect sugar accumulation.Compared with low-sugar genotype,high-sugar genotype had higher chlorophyll content,expression level of SPS family genes,sugar content and activities of sucrose metabolism-related enzymes,which indicated that high-sugar genotype had more active sucrose metabolism in sugarcane leaves.

关 键 词：甘蔗 蔗糖磷酸合成酶 蔗糖代谢 基因表达 

分 类 号：S566.1[农业科学—作物学]