多柔比星对兔眼准分子激光角膜切削术后角膜上皮下混浊(Haze)的影响  

Effect of doxorubicin on subepithelial opacity(Haze)after photorefractive keratectomy

作　　者：李娜 樊郑军 石芊 王静 隋艳来 LI Na;FAN Zhengjun;SHI Qian;WANG Jing;SUI Yanlai(Department of Ophthalmology,the Sixth Medical Center of PLA General Hospital,Beijing 100048,China)

机构地区：[1]中国人民解放军总医院第六医学中心眼科,北京市100084

出　　处：《眼科新进展》2020年第7期617-618,624,共3页Recent Advances in Ophthalmology

摘　　要：目的通过动物实验初步观察应用多柔比星对准分子激光角膜切削术(photorefractive keratectomy,PRK)术后角膜上皮下混浊(Haze)的影响,评估其抑制PRK术后Haze的有效性和安全性,旨在探讨PRK术中多柔比星替代丝裂霉素C应用的可行性。方法新西兰白兔10只(20眼),其中右眼为多柔比星治疗的实验组,左眼为丝裂霉素C治疗的对照组,两组兔眼均行PRK激光切削校正-8.00 D后,分别用0.2 g·L-1多柔比星或0.2 g·L-1丝裂霉素C的棉片处理,分别于术后1 d、1周、2周、3周、4周在裂隙灯下观察角膜上皮愈合及Haze情况。结果术后1周,两组所有兔眼角膜上皮均已完全愈合。实验组3眼术后1 d即出现角膜基质片状炎性浸润灶,其中2眼治疗后角膜基质炎性浸润灶消退,另1眼形成浅层斑翳;实验组其余7眼及对照组10眼均未见Haze。结论多柔比星虽然可能引起角膜基质非感染性炎症反应,但在抑制PRK术后细胞增殖方面或具有与丝裂霉素C类似效果。Objective To observe the effects of doxorubicin on Haze after photorefractive keratectomy(PRK)in animal experiments,and evaluate the efficacy and safety of doxorubicin in inhibiting Haze after PRK in order to explore the possibility of doxorubicin replacing mitomycin in PRK.Methods In 10 rabbits,the 10 right eyes were treated with doxorubicin(doxorubicin experimental group),while the 10 left eyes was treated with mitomycin C(mitomycin C group).After PRK laser ablation was performed for correcting as-8.0D,the cotton slices soaked with 0.2 g·L-1 doxorubicin or 0.2 g·L-1 mitomycin C were placed in the central ablation area respectively.The corneal epithelial healing and Haze were observed and summarized under slit lamp at 1 day,1 week,2 weeks,3 weeks and 4 weeks after operation.Results There was no delay in corneal epithelial healing in all eyes.In the doxorubicin experimental group,patchy inflammatory infiltration of corneal stroma occurred in 3 eyes one day after operation.Among them,2 eyes of the corneal stromal infiltration foci subsided after treatment,and the other eye formed superficial plaque.Haze did not occur in the remaining 7 eyes in the doxorubicin experimental group and 10 eyes in the mitomycin C group.Conclusion Although doxorubicin may cause non-infectious inflammation of corneal stroma,it may have similar effects with mitomycin C in inhibiting cell proliferation after PRK.

关 键 词：多柔比星 准分子激光角膜切削术(PRK) 角膜上皮下混浊(Haze) 丝裂霉素C 

分 类 号：R772.2[医药卫生—眼科]