ZEB1-AS1在子宫内膜癌中的表达及其与临床病理特征的关系  

Expression of ZEB1-AS1 Expression in Endometrial Carcinoma and Its Correlation with Clinicopathological Features

作　　者：宋娇 王利[2] SONG Jiao;WANG Li(Department of Gynecology,Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China;Department of Gynecology,Zhengzhou First People's Hospital,Zhengzhou 450000,China)

机构地区：[1]郑州大学第三附属医院妇科,郑州450000 [2]郑州市第一人民医院妇科,郑州450000

出　　处：《肿瘤防治研究》2020年第6期457-461,共5页Cancer Research on Prevention and Treatment

摘　　要：目的探讨ZEB1-AS1在子宫内膜癌中的表达及其与临床病理特征的关系。方法qRT-PCR法检测195对子宫内膜癌-癌旁正常组织标本中ZEB1-AS1及ZEB1的表达量;分析ZEB1-AS1及ZEB1的表达量与子宫内膜癌患者临床病理特征的关系;MTT、Transwell迁移实验、侵袭实验分别检测过表达ZEB1-AS1稳转细胞系增殖、迁移和侵袭能力的变化。结果ZEB1-AS1与ZEB1在子宫内膜癌中均高表达,且两者成正相关(P<0.05),两者分别高表达均可缩短患者生存期(均P<0.05);ZEB1-AS1表达与患者肿瘤家族史、病理分级、病理分型、化疗、肿瘤分期、T分期、M分期和HPV感染相关(均P<0.05),ZEB1表达与患者肿瘤家族史、病理分级、肿瘤分期、T分期、N分期和M分期相关(均P<0.05);过表达ZEB1-AS1可促进子宫内膜癌细胞增殖、迁移和侵袭(均P<0.05)。结论ZEB1-AS1及ZEB1在子宫内膜癌中高表达,可促进子宫内膜癌转移和侵袭并提示不良预后。Objective To explore the expression of ZEB1-AS1 in endometrial cancer(EC),and its correlation with clinicopathological features.Methods The expression of ZEB1-AS1 and ZEB1 in 195 pairs of endometrial cancer and paracancerous tissues were detected by qRT-PCR analysis.We analyzed the correlation of ZEB1-AS1 and ZEB1 expression with the clinical characteristics of EC patients.MTT assay,Transwell migration assay and invasion assay were used to detect the proliferation,migration and invasion of EC cell lines after ZEB1-AS1 overexpression.Results ZEB1-AS1 and ZEB1 were upregulated in EC tissues(P<0.05).The high expression level of ZEB1-AS1 and ZEB1 were positively correlated and could shorten the overall survival time of EC patients(P<0.05).The high expression levels of ZEB1-AS1 was correlated with family history,pathology grade,pathological type,chemotherapy,clinical stage,T stage,M stage and HPV infection of EC patients(P<0.05).The high expression levels of ZEB1 was correlated with family history,pathology grade,clinical stage,T stage,N stage and M stage(P<0.05).Overexpression of ZEB1-AS1 promoted the proliferation,migration and invasion of EC cell lines(all P<0.05).Conclusion Both ZEB1-AS1 and ZEB1 are upregulated in EC tissues and could promote the metastasis,invasion of endometrial cancer and indicate the poor prognosis.

关 键 词：ZEB1-AS1 ZEB1 子宫内膜癌 预后 病理分型 

分 类 号：R737.33[医药卫生—肿瘤]