荧光定量检测试纸检测饲料中黄曲霉毒素B1  

Detection of Aflatoxin B1 in Feed by Fluorescence Quantitative Test Paper

作　　者：王虎 WANG Hu(Liaoning Agricultural Development Service Center,Shenyang 110032,China)

机构地区：[1]辽宁省农业发展服务中心,沈阳110032

出　　处：《饲料博览》2020年第5期43-46,共4页Feed Review

摘　　要：研究建立了饲料中黄曲霉毒素B_1荧光定量检测方法。饲料样品经70%甲醇溶液提取,过滤后,用0.01 mol·L^-1pH 7.4 PBS复溶,结果表明,黄曲霉毒素B1在5~80μg·mL^-1浓度范围内线性关系良好,r^2=0.998。以配合饲料、浓缩饲料、预混料为添加基质,其回收率在80%~120%,检出限为5μg·mL^-1。结果显示,本方法灵敏度高,操作简单,结果准确,可满足饲料中黄曲霉毒素B1的分析检测。In this study,we established a fluorescent quantitative method for the detection of aflatoxin B1 in feed.Feed samples were extracted with 70%methanol solution,filtered and re dissolved with 0.01 mol·L^-1 pH 7.4 PBS.The results showed that aflatoxin B1 had a good linear relationship in the concentration range of 5-80μg·mL^-1,r2=0.998.The recovery was 80%-120%and the detection limit was 5μg·mL-1.Conclusion:the method has high sensitivity,simple operation and accurate results,and can be used for the determination of aflatoxin B1 in feed.

关 键 词：黄曲霉毒素B1 饲料 荧光定量检测试纸 

分 类 号：S816[农业科学—饲料科学]