两阶段调控葡萄糖质量浓度强化赤藓糖醇发酵的研究  

Two-stage regulation of glucose concentration for erythritol production

作　　者：刘金龙[1,2] 孙锡友 赵国群 王勇[1] 白敬 孙旭 LIU Jinlong;SUN Xiyou;ZHAO Guoqun;WANG Yong;BAI Jing;SUN Xu(College of Bioscience and Bioengineering,Hebei University of Science and Technology,Shijiazhuang 050000,China;Research Center for Fermentation Engineering of Hebei,Shijiazhuang 050000,China)

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050000 [2]河北省发酵工程技术研究中心,河北石家庄050000

出　　处：《中国酿造》2020年第6期88-92,共5页China Brewing

基　　金：河北省自然科学基金项目(C2016208052);河北科技大学五大平台开放基金(1182049)。

摘　　要：该研究在5 L发酵罐水平上研究了不同初始葡萄糖质量浓度对解脂耶氏酵母(Yarrowia lipolytica)JZ-204生长和发酵产赤藓糖醇的影响。结果表明,100 g/L初始葡萄糖质量浓度有利于菌体生长,高初始葡萄糖质量浓度(300 g/L和400 g/L)有利于赤藓糖醇合成。基于此,提出两阶段葡萄糖质量浓度调控策略,即0 h时以初始葡萄糖质量浓度为100 g/L,22 h后通过补加葡萄糖使总糖量达300 g/L进行发酵。结果表明,与分批发酵相比(100 g/L、200 g/L、300 g/L、400 g/L),采用该调控策略,赤藓糖醇产量达到最高水平92.66 g/L,分别比分批发酵提高了1347.81%、84.54%、14.66%、7.57%;生产强度达到最高的0.48 g/(L·h),分别比分批发酵提高了300%、37.14%、29.73%、20.00%。该调控策略为赤藓糖醇的高效发酵合成提供参考。The effect of different initial glucose concentration on the growth of Yarrowia lipolytica and erythritol biosynthesis was compared.Results showed that glucose concentration 100 g/L was beneficial to the cell growth of Y.lipolytica JZ-204,high glucose concentration(300 g/L and 400 g/L)contributed to the production of erythritol.Thus,a two-stage fermentation was conducted based on the control of glucose concentration,the initial glucose concentration was 100 g/L at 0 h,and extra glucose was added to increase the total glucose concentration to 300 g/L at 22 h.Results showed that compared with batch fermentation(100 g/L,200 g/L,300 g/L,400 g/L),the production of erythritol reached the highest level of 92.66 g/L under the control strategy,which was 1347.81%,84.54%,14.66%and 7.57%higher than that achieved in batch fermentations,respectively.The production of erythritol reached 0.48 g/(L·h),which was 300%,37.14%,29.73%and 20.00%higher than that obtained from batch fermentation,respectively.The control strategy will provide a reference for high-efficiency production of erythritol.

关 键 词：解脂耶氏酵母 赤藓糖醇 葡萄糖质量浓度 比生长速率 比合成速率 

分 类 号：Q815[生物学—生物工程]