老龄大鼠术后认知功能障碍时海马HSP70表达的变化  

Changes in expression of hippocampal HSP70 during postoperative cognitive dysfunction in aged rats

作　　者：蒋素芳[1] 刘雅[1] 康荣田[1] Jiang Sufang;Liu Ya;Kang Rongtian(Department of Anesthesiaology,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院麻醉科,石家庄050000

出　　处：《中华麻醉学杂志》2020年第3期309-311,共3页Chinese Journal of Anesthesiology

基　　金：河北省自然科学基金(H2018206191);河北省医学科学研究重点课题(20180305)。

摘　　要：目的评价老龄大鼠术后认知功能障碍时海马热休克蛋白70(HSP70)表达的变化。方法清洁级健康雄性老龄SD大鼠12只,18月龄,体重500~650 g,采用随机数字表法分为2组(n=6):老龄对照组(O组)和老龄手术组(OS)。另取清洁级健康雄性成年SD大鼠12只为对比,采用随机数字表法分为2组(n=6):成年对照组(A组)和成年手术组(AS组)。AS组和OS组行剖腹探查术。于术后第3天行Morris水迷宫实验,随后处死大鼠,分离海马组织,采用Western blot法检测HSP70的表达,ELISA法检测IL-1β和TNF-α含量。结果与A组比较,AS组海马HSP70表达上调(P<0.05),其余指标差异无统计学意义(P>0.05);与O组比较,OS组逃避潜伏期延长,穿越平台次数减少,海马IL-1β和TNF-α含量升高,HSP70表达上调(P<0.05);与AS组比较,OS组逃避潜伏期延长,穿越平台次数减少,海马IL-1β和TNF-α含量升高(P<0.05),HSP70表达差异无统计学意义(P>0.05)。结论老龄大鼠术后认知功能障碍时海马HSP70表达上调,有助于抑制炎症反应,发挥内源性神经保护效应。Objective To evaluate the changes in the expression of hippocampal HSP70 during postoperative cognitive dysfunction in aged rats.Methods Twelve pathogen-free healthy male Sprague-Dawley rats,aged 18 weeks,weighing 500-650 g,were divided into 2 groups(n=6 each)using a random number table method:old control group(group O)and old surgery group(group OS).Another 12 pathogen-free healthy male Sprague-Dawley rats served as control and divided into 2 groups(n=6 each)according to a random number table method:adult control group(group A)and adult surgery group(group AS).Exploratory laparotomy was performed in group AS and group OS.Morris water maze test was performed at 3 days after operation,and then the rats were sacrificed,and the hippocampi were removed for determination of the expression of heat shock protein 70(HSP70)(by Western blot)and contents of interleukin-1beta(IL-1β)and tumor necrosis factor-alpha(TNF-α)(by enzyme-linked immunosorbent assay).Results Compared with group A,the expression of HSP70 was significantly up-regulated(P<0.05),and no significant change was found in the other indices in group AS(P>0.05).Compared with group O,the escape latency was significantly prolonged,the frequency of crossing platform was decreased,the contents of IL-1βand TNF-αwere increased,and the expression of HSP70 was up-regulated in group OS(P<0.05).Compared with group AS,the escape latency was significantly prolonged,the frequency of crossing platform was decreased,the contents of IL-1βand TNF-αwere increased(P<0.05),and no significant change was found in the expression of HSP70 in group OS(P>0.05).Conclusion HSP70 expression in hippocampi is up-regulated during postoperative cognitive dysfunction and is helpful in inhibiting the inflammatory response and exerting endogenous neuroprotective effect in aged rats.

关 键 词：HSC70热休克蛋白质类 认知功能障碍 老年人 

分 类 号：R749.1[医药卫生—神经病学与精神病学]