电子烟气溶胶提取物细胞增殖毒性研究  

Cell proliferation toxicity of electronic cigarette aerosol extract in vitro

作　　者：李典[1] 李志华[1] 梁俊[1] 黃忠辉 徐石磊[1] 陆漓 陆彩玲 LI Dian;LI Zhi-hua;LIANG Jun;HUANG Zhong-hui;XU Shi-lei;LU Li;LU Cai-ling(Technical Center of China Tobacco Guangxi Indus trial CO.,LTD,Nanning,Guangxi 530001,China;不详)

机构地区：[1]广西中烟工业有限责任公司技术中心,广西南宁530001 [2]广西医科大学公共卫生学院

出　　处：《环境与健康杂志》2019年第9期782-785,共4页Journal of Environment and Health

基　　金：广西中烟工业有限责任公司项目(GXZYCX2017C016)。

摘　　要：目的探讨电子烟气溶胶提取物的体外急性毒性,为电子烟的评价提供依据。方法电子烟经吸烟机器人抽吸,产生的烟气气溶胶经A(含分子筛滤嘴)、B(不含分子筛滤嘴)不同传输装置后经剑桥滤片捕获,将MEM、DMSO提取剑桥滤片吸附的烟气气溶胶后所得的水、脂溶性气溶胶提取液及烟油原液分别与中国仓鼠卵巢细胞(CHO-K1)、人支气管上皮样细胞(HBE)、人胚肺成纤维细胞(MRC-5)三株细胞共孵育24 h,以CCK-8检测细胞活性。结果烟油原液对各细胞增殖抑制呈浓度依赖性升高趋势,烟油浓度在21.620~108.1 mg/ml时对MRC-5和CHO-K1产生细胞增殖毒性,差异有统计学意义(P<0.05),烟油浓度在10.810~108.1 mg/ml时对HBE产生细胞增殖毒性,差异有统计学意义(P<0.05);各细胞的半数抑制浓度(IC50)依次为39.35 mg/ml(MRC-5)<42.69 mg/ml(CHO-K1)<55.91 mg/ml(HBE)。B传输装置水溶性提取剂>1.31 mg/ml(用烟油浓度标定)时具有抑制作用,差异有统计学意义(P<0.05);在检测浓度范围内,各细胞的IC50依次为1.24 mg/ml(CHO-K1)<1.91 mg/ml(MRC-5)<2.08 mg/ml(HBE);IC50比烟油原液低一个数量级,差异有统计学意义(P<0.05)。A传输装置的水、脂提取物及B传输装置的脂提取物对细胞增殖无抑制作用。结论烟油原液可抑制细胞增殖,加热后产物(即电子烟气溶胶提取物)的细胞增殖抑制能力与传输装置的构造有关,含滤嘴的A装置产生的电子烟气溶胶未见细胞增殖毒性,普通滤嘴B装置的水溶性提取物表现出细胞增殖毒性,且比烟油原液高。3种细胞中,CHO-K1对电子烟气溶胶的毒性最敏感,MRC-5对烟油的毒性最敏感。Objective To investigate cell proliferation toxicity of e-cigarette aerosol extraction in vitro.Methods The e-cigarette aerosol driven by smoking robot was captured by the Cambridge filter paper then extracted by MEM and DMSO,respectively.Before being captures,the e-cigarette aerosol went through a device with or without filter.Three cell lines(CHO-K1,HBE and MRC-5)were incubated with the e-liquid of electronic cigarette and two kinds of aerosol extractions from two different devices for 24 h and cell viability were detected by CCK-8.Resuts The half-inhibitory concentration(IC50,mg/ml)of the e-cigarette liquid was 39.35(MRC-5)<42.69(CHO-K1)<55.91(HBE).The water-soluble aerosol(MEM)from B transmission device without filter inhibited cell proliferation at more than 1.31 mg/ml,with its half-inhibitory concentration(IC50,mg/mL)among different cell lines as 1.24(CHO-K1)<1.91(MRC-5)<2.08(HBE),which was about one order of magnitude lower than ecigarette liquid.No proliferation inhibition was observed in either extraction from A transmission device with filter.Conclusions The e-cigarette liquid inhibites cell proliferation.However,the proliferation inhibition of the aerosol extractions seems to depend on the transmission devices.MEM aerosol extraction from transmission without filter shows the most severe proliferation toxicity among the four different aerosol extractions compared with e-cigarette liquid.No proliferation inhibition is observed in extraction from A transmission device with filter.Among the three cells,CHO-K1 is more susceptible to the toxicity of e-cigarette aerosols while MRC-5 is more sensitive to the toxicity of e-cigarette liquid.

关 键 词：电子烟气溶胶 水溶性提取物 脂溶性提取物 细胞增殖毒性 半数抑制浓度 

分 类 号：R994.6[医药卫生—毒理学]