小鼠局部淋巴结试验方法盲样法评价  

A murine local lymph node assay:Daicel adenosine triphosphate test on eleven blind samples in BALB/c mice

作　　者：张丽霞[1] 石莹[1] 段链[1] 王超[1] 张少平[1] 阮鸿洁[1] 张明[1] 袁园 王翀[1] ZHANG Li-xia;SHI Ying;DUAN Lian(National Institute of Environmental Health,Chinese Center for Disease Control and Prevention,Beijing 100021,China)

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《中国公共卫生》2020年第6期901-904,共4页Chinese Journal of Public Health

摘　　要：目的应用小鼠局部淋巴结试验(LLNA:DA)方法检测11个盲样的致敏性,验证BALB/c小鼠可以替代OECD指南中的CBA/J小鼠。方法选择11个未知样品作为受试物,受试物现用现配。实验前1 h先在小鼠双耳后涂抹1%SLS,然后涂抹受试物。分别在第1、2、3天及第7天涂抹,第8天取双耳片和耳后淋巴结,用ATP荧光检测仪测定ATP含量,计算刺激指数SI和EC1.8。结果与经典豚鼠试验数据库比较,本次研究中2只、3只和4只动物的实验结果与数据库一致率分别为82%、73%和91%;与LLNA实验结果比较,2只、3只和4只动物的实验结果与数据库一致率分别为64%、55%和82%。结论无论与经典豚鼠实验数据库比较,还是与LLNA数据库比较,4只动物的实验结果更敏感,更准确,使用BALB/c小鼠可以替代OECD指南中的CBA/J小鼠;4只动物均可以用于致敏物质的初筛。Objective To evaluate the allergenicity of 11 blind samples with murine local lymph node assay:Daicel adenosine triphosphate(LLNA:DA)test method and to verify whether the CBA/J mice could be substituted with BALB/c mice in LLNA:DA test recommended by Organization for Economic Co-operation and Development(OECD)guideline.Methods Three dosages were determined for each of the 11 unknown chemical materials with pre-test.Four specific pathogen free(SPF)BALB/c mice of 8–12 weeks old were used for each dosage group.One hour after smearing of 1%sodium lauryl sulfates(SLS),the various dosages of bland samples dissolved in acetone(ACE),dimethyl sulfoxide(DMSO),or ACE and olive oil(4:1 v/v)were administered to the back of the mice′s ears four times in a week(on the 1st,2nd,3rd and 7th day).Specimens of ear tissues and lymph nodes of the mice were collected one day after the last administration.Adenosine triphosphate(ATP)content was measured with ATP fluorescence detector and the stimulation index SI and EC1.8 were determined.Results Compared with those from referral datasets of classic guinea pig test,the consistency rates were 82%,73%and 91%for the results the tests with 2,3 and 4 mice;while,between the results of LLNA and LLNA:DA,the consistency rates were 64%,55%,and 82%for the results the tests with 2,3 and 4 mice,respectively.Conclusion Compared with both the classic guinea pig experimental database and the LLNA database,the results of the tests with four mice were more sensitive and more accurate,indicating that CBA/J mice could be substituted with BALB/c mice in LLNA:DA test recommended by OECD guideline.

关 键 词：致敏性 小鼠局部淋巴结试验 刺激指数 EC1.8 化妆品原料 

分 类 号：R114[医药卫生—卫生毒理学]