长链非编码RNA SNHG3在食管鳞状细胞癌中的表达及其对ECA-109细胞迁移和侵袭的影响  

Expression of long non-coding RNA SNHG3 in esophageal squamous cell carcinoma and its effect on the migration and invasion of ECA-109 cells

作　　者：郭晓锋 朱曼 许广辉[3] 王文光[3] 郝安林[1] Xiaofeng Guo;Man Zhu;Guanghui Xu;Wenguang Wang;Anlin Hao(Department of Thoracic Surgery,Anyang Tumor Hospital,Anyang 455000,China;Clinical Laboratory,Anyang Tumor Hospital,Anyang 455000,China;Department of Thoracic Surgery,Henan Province Chest Hospital,Zhengzhou 450000,China)

机构地区：[1]安阳市肿瘤医院胸外科,河南省安阳市455000 [2]安阳市肿瘤医院检验科,河南省安阳市455000 [3]河南省胸科医院胸外科

出　　处：《中国肿瘤临床》2020年第12期595-600,共6页Chinese Journal of Clinical Oncology

基　　金：河南省科技攻关计划(编号:172102310116)资助。

摘　　要：目的:探究长链非编码RNA SNHG3在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)组织中的表达及其对ECA-109细胞迁移和侵袭的影响。方法:收集安阳市肿瘤医院2011年6月到2014年6月收治的60例ESCC患者癌及对应癌旁组织样本,采用qRT-PCR检测ESCC癌与癌旁组织、ESCC细胞ECA-109和人正常食管上皮细胞HEEC中lncRNA SNHG3的表达水平,分析ESCC患者组织中lncRNA SNHG3的表达与ESCC患者临床特征的关系。采用siRNA-SNHG3质粒转染ECA-109细胞,以敲降lncRNA SNHG3的表达水平。采用Kaplan-Meier法分析lncRNA SNHG3表达与患者预后的关系。采用Transwell实验检测ECA-109细胞的迁移和侵袭能力。采用qRT-PCR和Western blot实验检测ECA-109细胞中SNAIL和TWIST的mRNA及蛋白的表达水平。结果:ESCC肿瘤组织中lncRNA SNHG3的表达水平显著高于癌旁组织(P<0.05),ECA-109细胞中lncRNA SNHG3的表达水平显著高于人正常食管上皮细胞HEEC(P<0.05)。ESCC患者组织中lncRNA SNHG3的表达水平与肿瘤分化程度、TNM分期及淋巴结转移情况显著相关(P<0.05)。siRNA-SNHG3质粒转染ECA-109细胞后,lncRNA SNHG3的表达水平显著降低(P<0.05)。lncRNA SNHG3的高表达与ESCC患者预后不良显著相关。敲降lncRNA SNHG3后,ECA-109细胞的迁移和侵袭能力显著降低(P<0.05),SNAIL和TWIST的mRNA及蛋白的表达水平显著降低(P<0.05)。结论:lncRNA SNHG3在ESCC肿瘤组织和细胞中高表达,通过调控SNAIL和TWIST蛋白的表达,促进ECA-109细胞的迁移和侵袭。Objective:To investigate the expression of long non-coding RNA(lncRNA)SNHG3 in esophageal squamous cell carcinoma(ESCC)and its effect on the migration and invasion of ECA-109 cells.Methods:Samples of tumor and corresponding para-carcinoma tissues were collected from 60 patients with ESCC,who were enrolled in Anyang Tumor Hospital from June 2011 to June 2014.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of lncRNA SNHG3 in ESCC tumor and para-carcinoma tissues,ECA-109 ESCC cells,and HEEC human normal esophageal epithelial cells.The correlation between lncRNA SNHG3 expression and clinical characteristics of patients with ESCC was assessed.ECA-109 cells were transfected with si RNA-SNHG3 plasmids to knockdown lnc RNA SNHG3 expression.The correlation between lnc RNA SNHG3 expression and patient prognosis was determined by Kaplan-Meier analysis.Transwell assay was used to investigate the migration and invasion abilities of ECA-109 cells.SNAIL and TWIST mRNA and protein levels in ECA-109 cells were detected by q RT-PCR and Western blot assay,respectively.Results:The expression level of lnc RNA SNHG3 was significantly higher in ESCC tumor tissues compared to that in pericarcinomatous tissues and in ECA-109 cells compared to that in HEEC cells(both P<0.05).Lnc RNA SNHG3 expression in ESCC cells was significantly correlated with tumor differentiation,TNM stage,and lymph node metastasis(P<0.05).LncRNA SNHG3 expression in ECA-109 cells decreased significantly upon transfection with the siRNA-SNHG3 plasmid(P<0.05).High expression of lncRNA SNHG3 was significantly correlated with poor prognosis in patients with ESCC.After lncRNA SNHG3 knockdown,the migration and invasion of ECA-109 cells and the m RNA and protein expression levels of SNAIL and TWIST were reduced significantly(P<0.05).Conclusions:Lnc RNA SNHG3 is highly expressed in ESCC tumor tissues and cells and promotes the migration and invasion of ECA-109 cells by regulating the expression of SNAIL and TWIST proteins.

关 键 词：长链非编码RNA SNHG3 食管鳞状细胞癌 迁移 侵袭 

分 类 号：R735.1[医药卫生—肿瘤]