a-1,2岩藻糖基转移酶Ⅱ在肺鳞癌组织中的表达及对肺鳞癌细胞增殖的影响  

Expression of a-1,2 fucosyltransferase II in lung squamous cell carcinoma and its effect on proliferation of lung squamous cell carcinoma cells

作　　者：王维杰 范赛荣 张光辉 孙海婷 施国富 马璇 朱成楚 WANG Weijie;FAN Sairong;ZHANG Guanghui;SUN Haiting;SHI Guofu;MA Xuan;ZHU Chengchu(Department of Thoracic Surgery,the Affiliated Xiangshan Hospital of Wenzhou Medical University,Ningbo 315700,China;School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou 325035,China;Department of Thoracic Surgery,the Affiliated Taizhou Hospital of Wenzhou Medical University,Taizhou 317000,China)

机构地区：[1]温州医科大学附属象山医院胸外科,浙江宁波315700 [2]温州医科大学检验医学院生命科学学院,浙江温州325035 [3]温州医科大学附属台州医院胸外科,浙江台州317000

出　　处：《温州医科大学学报》2020年第8期662-665,共4页Journal of Wenzhou Medical University

基　　金：象山县科技计划项目(2019c6002);温州市科技计划项目(Y20180221)。

摘　　要：目的:探讨a-1,2岩藻糖基转移酶Ⅱ(FUT2)在肺鳞癌中的表达情况及其对肺鳞癌细胞增殖的影响。方法:应用UALCAN数据库分析FUT2在临床肺鳞癌患者组织中的差异表达及其与肺鳞癌患者临床TNM分期、年龄及性别因素的相关性;采用瞬时转染技术,将FUT2的shRNA质粒转染进H226细胞株中构建FUT2低表达的肺鳞癌细胞模型;利用CCK-8、平板克隆及Western blot实验检测FUT2对肺鳞癌细胞的生长、单克隆细胞团形成及PCNA蛋白表达的影响。结果:FUT2在肺鳞癌尤其是肺鳞癌TNM早中期呈现高表达趋势(P<0.05),而与年龄和性别无关。低表达FUT2可以抑制肺鳞癌细胞的生长速率和单克隆细胞团的形成数,同时,可以抑制肺鳞癌细胞PCNA蛋白的表达水平,差异均有统计学意义(P<0.05)。结论:FUT2在肺鳞癌中高表达且促进肺鳞癌细胞的增殖能力,有望成为肺鳞癌的早期诊断指标。Objective: To investigate the expression of a-1, 2 fucosyltransferase Ⅱ(FUT2) in human lung squamous cell carcinoma(LUSC) and FUT2 effect on the cell proliferation of LUSC. Methods: UALCAN database was used to analyze the expression and clinical significance of FUT2 in lung squamous carcinoma;the sh RNA plasmid of FUT2 was transferred into LUSC’s cell line(H226) by transient transfer technique to construct the knockdown expression of FUT2;CCK-8, colony formation and Western blot were used to investigate the effect of FUT2 on cell proliferation of LUSC-cell growth, monoclonal cell mass formation and the expression of proliferator PCNA protein. Results: The expression of FUT2 in LUSC’s tumor tissue was higher than that in normal control tissue, especially in the early and middle stages(P<0.05). But the expression of FUT2 had no relations with LUSC patients’ age and sex. In addition, the lower expression of FUT2 inhibited the growth and formation of monoclonal cell clusters as well as repressed the expression of PCNA protein in LUSC’s cell line, which all has statistical significance(P<0.05). Conclusion: FUT2 is highly expressed in LUSC and could promote the cell proliferation of LUSC, which might become a diagnostic index of LUSC.

关 键 词：a-1 2岩藻糖基转移酶Ⅱ 肺鳞癌 细胞增殖 

分 类 号：R734.2[医药卫生—肿瘤]