Genotoxins exaggerate the stressed state of aneuploid embryonic stem cells via activation of autophagy  

作　　者：Meili Zhang Rong Xiao Guang Liu Yue Huang 

机构地区：[1]State Key Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100005,China [2]Department of Medical Genetics,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100005,China

出　　处：《Science China(Life Sciences)》2020年第7期1026-1036,共11页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China (2016YFA0100103 to Y.H.);CAMS Innovation Fund for Medical Sciences (2016-I2M-3-002 to Y.H. and M.Z.);National Natural Science Foundation of China (31701193 to M. Z.)。

摘　　要：The cellular consequences of aneuploidy are largely dependent on the cell types examined. Aneuploid yeasts and mouse embryonic fibroblasts exhibit cell proliferation defects and can be selectively inhibited by compounds that cause proteotoxic or energy stress. By contrast, most aneuploid pluripotent stem cells proliferate rapidly and reach higher saturation densities. The responses of aneuploid pluripotent stem cells to the stress-inducing compounds remain uncharacterized. Here, we tested the response of aneuploid embryonic stem cells to several compounds that caused proteotoxic, energy and genotoxic stress using previously established mouse embryonic stem cell lines trisomic for chromosome 6, 8, 11, or 15. Not all trisomic embryonic stem cells were selectively inhibited by compounds that cause proteotoxic or energy stress. However, most of these cells exhibited increased sensitivity to genotoxins. They displayed elevated DNA damage response as characterized by increased γH2A.X foci under genotoxic stress. Further investigations indicated that elevated autophagy levels might contribute to the increased cytotoxic effects of genotoxins on trisomic embryonic stem cells. Our study laid the foundation for eliminating aneuploidy that might be an effective approach for controlling cancer progression.

关 键 词：ANEUPLOIDY embryonic stem cells GENOTOXINS AUTOPHAGY 

分 类 号：R346[医药卫生—基础医学]