二氯苯醌对斑马鱼胚胎发育及超氧化物歧化酶的影响  

The effect of 2,6⁃DCBQ on the development and superoxide dismutase of zebrafish embryo

作　　者：滕晓强 陆宏峰 郭心月 高畅 罗钦方 张瑜 洪华嫦[1] 孙洪杰[1] TENG Xiaoqiang;LU Hongfeng;GUO Xinyue;GAO Chang;LUO Qinfang;ZHANG Yu;HONG Huachang;SUN Hongjie(College of Geography and Environmental Science,Zhejiang Normal University,Jinhua 321004)

机构地区：[1]浙江师范大学地理与环境科学学院,金华321004

出　　处：《环境科学学报》2020年第7期2659-2664,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.21707123);浙江省公益应用项目(No.LGF19B070007)。

摘　　要：2,6-二氯-1,4-苯醌(2,6-dichloro-1,4-benzoquinone, 2,6-DCBQ)作为一种新型消毒副产物,因其高检出率及高含量的特点而备受人们关注.本研究以斑马鱼胚胎为实验材料探究了2,6-DCBQ的发育毒性.结果发现:斑马鱼胚胎在8~24 hpf(器官发育形成时期)对2,6-DCBQ暴露最为敏感,死亡率最高;2,6-DCBQ安全浓度为27.15μg·L^-1,属于极度危险外源化学物.在本实验中,2,6-DCBQ对斑马鱼的生长没有显著影响,但高浓度(100和150μg·L^-1)2,6-DCBQ会显著增加斑马鱼胚胎及仔鱼的畸形率.在实验过程中还发现,高浓度(≥80μg·L^-1)2,6-DCBQ会明显抑制斑马鱼胚胎的心率.此外,≥30μg·L^-1 2,6-DCBQ可提高超氧化物歧化酶(Superoxide dismutase, SOD)的活性,并影响Cu/ZnSOD和MnSOD的正常转录.本研究较全面地探究了2,6-DCBQ对斑马鱼早期发育阶段的发育毒性,可为2,6-DCBQ的毒性研究提供可靠证据.2,6⁃dichloro⁃1,4⁃benzoquinone(2,6⁃DCBQ),as an emerging disinfection by⁃products,has attracted much attentions due to frequently detected and high levels.In this study,zebrafish embryo was employed to explore the toxic effect of 2,6⁃DCBQ on the developmental performance of zebrafish embryo.Our results clearly indicated that zebrafish embryo at 8~24 hpf was most sensitive to 2,6⁃DCBQ,and more embryos died at 8~24 hpf.The safe concentrations of 2,6⁃DCBQ was 27.15μg·L^-1,and thus 2,6⁃DCBQ is classified as the extremely dangerous pollutant.In the present study,2,6⁃DCBQ had no significant effect on zebrafish growth,whereas higher concentrations(100 and 150μg·L^-1)of 2,6⁃DCBQ obviously inhibited zebrafish embryo heart beating.Besides,in this study,over 30μg·L^-1 of 2,6⁃DCBQ significantly elevated superoxide dismutase(SOD)activity and disrupted the transcriptional levels of Cu/ZnSOD and MnSOD.Our results comprehensively demonstrate the toxic effect of 2,6⁃DCBQ on developmental performance of zebrafish embryos,providing reliable evidence for 2,6⁃DCBQ toxicity.

关 键 词：二氯苯醌 斑马鱼胚胎 发育毒性 SOD活性 SOD转录 

分 类 号：X171.5[环境科学与工程—环境科学]