赤霉素缺失型番茄果实抗冷性变化  

Chilling resistance of gibberellin-deficiency tomato fruits

作　　者：黄笑非 屈彤彤 丁洋 雷彤彤 张洁[1] 唐选明[1] HUANG Xiaofei;QU Tongtong;DING Yang;LEI Tongtong;ZHANG Jie;TANG Xuanming(Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Beijing Wuzi University,School of Logistics/Beijing Key Laboratory of Logistics System and Technology,Beijing 101149,China)

机构地区：[1]中国农业科学院农产品加工研究所,北京100193 [2]北京物资学院,物流学院/北京市物流系统与技术重点实验室,北京101149

出　　处：《食品与发酵工业》2020年第13期99-106,共8页Food and Fermentation Industries

基　　金：国家重点研发专项(2016YFD0400105)。

摘　　要：为明确赤霉素在番茄果实抗冷调控中的作用地位,以绿熟期的野生型番茄果实及其赤霉素缺失型突变株(gib-3)为试材,4℃下贮藏,统计贮藏期间番茄果实冷害指数,观察细胞超微结构,测定细胞膜损伤强度、细胞膜脂酶、细胞壁降解酶以及抗氧化酶等活性的变化,研究赤霉素缺失型番茄果实抗冷特性。结果表明,与野生型番茄果实相比,赤霉素缺失型番茄果实冷害症状和超微结构破坏程度更为严重,丙二醛含量、离子渗透率升高,磷脂酶D、脂氧合酶、多聚半乳糖醛酸酶、果胶甲酯酶活性增大,细胞膜过氧化反应和细胞壁降解速度加快,果实细胞膜和细胞壁的完整性受到破坏;超氧化物歧化酶、过氧化氢酶、苯丙氨酸解氨酶和谷胱甘肽转移酶活性均降低,果实清除自由基能力降低,表明低温下赤霉素缺失不利于番茄果实抵御冷害。In order to identify the role of gibberellins(GAs)in the resistance of tomato fruits,the present study used the wild type(WT)and GA-deficient mutant(gib-3)tomato fruits at the mature green stage as raw materials and stored them at 4℃.The chilling injury(CI)index was measured,the ultra-structure of cells was observed,cell membrane damage intensity,as well as the activity changes of cell membrane lipase,cell wall degradation enzymes,and antioxidant enzymes of tomato fruits were determined.Results indicated that gib-3 tomato fruits showed more severe chilling symptoms and ultra-structural damage than WT tomato fruits.The MDA content,ion permeability and the activity of PLD,LOX,PG and PME of gib-3 tomato were increased.Cell membrane peroxidation and cell wall degradation of gib-3 tomato fruits accelerated,and their integrity of fruit cell membrane and cell wall was damaged.In addition,the activity of SOD,CAT,PAL and GST were decreased in gib-3 tomato fruits,and the ability of free radical scavenging reduced.According to the results,gibberellin deficiency at low temperatures was not good for tomato fruits to resist chilling injury.This study will provide a theoretical basis for the research on the chilling resistance mechanism of postharvest tomato fruits.

关 键 词：番茄果实 赤霉素缺失 冷害 抗冷能力 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]