Cloning and functional analysis of the calreticulin gene from the scleractinian coral Galaxea astreata  

作　　者：Yuanjia Huang Yanping Zhang Huipai Peng Jigui Yuan Li Liu 

机构地区：[1]Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes,Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China

出　　处：《Acta Oceanologica Sinica》2020年第6期58-64,共7页海洋学报（英文版）

基　　金：The National Marine Welfare Industry Research Project under contract No.201105012;the Guangdong Provincial Natural Science Foundation under contract No.S2011010000269;the Guangdong Marine Fishery Science and Technology Extension Project under contract No.A201308E02;the Guangdong Ocean University Scientific Research Start-up Fund for the Doctoral Program under contract No.R17066。

摘　　要：Calreticulin(CRT)is a highly conserved Ca2+-binding protein and chaperone in the endoplasmic reticulum,which mainly participates in adjusting calcium level and directing proper conformation of proteins.Here,we cloned the crt gene of the scleractinian coral Galaxea astreata,named Gacrt,and analysed its ability to drive bacterial agglutination.The full-length Gacrt cDNA consisted of 1792 nucleotides and contained a 77 bp 5’untranslated region(UTR),a 380 bp 3’UTR and a 1335 bp open reading frame(ORF)that encoded a 444 amino acid protein.The deduced peptide possessed a signal peptide domain,an endoplasmic reticulum retrieval signal sequence(KDEL),two potential calreticulin family signature motifs and a set of triplicate repeats.We also found that the recombinant GaCRT protein could promote agglutination of both the Gram-positive bacterium Micrococcus luteus and the Gram-negative bacterium Escherichia coli.These results show that the GaCRT protein can enhance bacterial agglutination,hinting that GaCRT is an immune-relevant molecule involved in host defense against bacterium.

关 键 词：Galaxea astreata CALRETICULIN bacterial agglutination 

分 类 号：Q953[生物学—动物学]