miR-34、MDM2、EPAS1在子宫内膜癌中的表达及与临床特征的相关性分析  

Expression of miR-34,MDM2 and EPAS1 in Endometrial Carcinoma and Their Correlation with Clinical Characteristics

作　　者：陈丽华[1] 朱婕曼 刘玉凤[1] 刘浩 马春星 张凡[2] 刘云春[1] CHEN Li-hua;ZHU Jie-man;LIU Yu-feng;LIU Hao;MA Chun-xing;ZHANG Fan;LIU Yun-chun(Department of Gynaecology and Obstetrics,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China;Department of pathology,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China)

机构地区：[1]河北北方学院附属第一医院妇产科,河北张家口075000 [2]河北北方学院附属第一医院病理科,河北张家口075000

出　　处：《解放军医药杂志》2020年第7期38-42,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：河北省医学科学研究重点课题计划(20180836)。

摘　　要：目的探究微小RNA-34(miR-34)、鼠双微基因2(MDM2)、内皮PAS1蛋白(EPAS1)在子宫内膜癌中的表达及与临床特征的相关性。方法选取2018年3月-2019年3月接受手术治疗的子宫内膜癌患者50例,自所有患者手术切除标本中取癌组织标本以及距离癌组织5 cm以上的癌旁组织待用,检测miR-34、MDM2、EPAS1相对表达量,观察miR-34、MDM2、EPAS1表达与子宫内膜癌患者肿瘤体积、病理分期、淋巴结转移情况、浸润程度、分化程度的关系。结果子宫内膜癌癌组织中miR-34表达低于癌旁组织,MDM2、EPAS1表达高于癌旁组织(P<0.01)。肿瘤体积≥5 cm^3患者miR-34表达低于肿瘤体积<5 cm^3患者,MDM2、EPAS1表达高于肿瘤体积<5cm^3患者(P<0.01)。病理分期为Ⅲ~Ⅳ的患者miR-34表达低于病理分期为Ⅰ~Ⅱ期的患者,MDM2、EPAS1表达高于病理分期为Ⅰ~Ⅱ期的患者(P<0.01)。有淋巴结转移的患者miR-34表达低于无淋巴结转移情况的患者,MDM2、EPAS1表达高于无淋巴结转移情况的患者(P<0.01)。浸润程度>1/2肌层的患者miR-34表达低于浸润程度≤1/2肌层的患者,MDM2、EPAS1表达高于浸润程度≤1/2肌层的患者(P<0.01)。低分化患者miR-34表达低于中分化、高分化患者,MDM2、EPAS1表达高于中分化、高分化患者,中分化患者miR-34表达低于高分化患者,MDM2、EPAS1表达高于高分化患者(P<0.01)。结论miR-34、MDM2、EPAS1在子宫内膜癌中呈现异常表达,且与肿瘤体积、病理分期、淋巴结转移情况、浸润程度、分化程度等临床病理参数具有一定相关性,与子宫内膜癌发生、演进过程密切相关。Objective To investigate the expression of microrna-34(miR-34),mouse double microgene 2(MDM2)and endothelial Pas1 protein(EPAS1)in endometrial carcinoma and their correlation with clinical features.Methods From March 2018 to March 2019,50 patients with endometrial carcinoma who received surgical treatment in our hospital were selected.The cancer tissue samples and the adjacent tissues more than 5 cm away from the cancer tissue were taken from all specimens of patients'surgical resection for preparation.The relative expression of miR-34,MDM2 and EPAS1 was detected,and the relationship between the expression of miR-34,MDM2,EPAS1 and tumor volume,pathological stage,lymph node metastasis,degree of invasion,degree of differentiation of endometrial carcinoma patients were observed.Results The expression of miR-34 in endometrial carcinoma was significantly lower than that in adjacent tissues,and the expression of MDM2 and EPAS1 was significantly higher than that in adjacent tissues(P<0.01).The expression of miR-34 in patients with tumor volume≥5 cm^3 was significantly lower than that in patients with tumor volume<5 cm^3,and the expression of MDM2 and EPAS1 was significantly higher than that in patients with tumor volume<5 cm^3(P<0.01).The expression of miR-34 in patients with pathological stageⅢandⅣwas significantly lower than that in patients with pathological stageⅠandⅡ,and the expression of MDM2 and EPAS1 was significantly higher than that in patients with pathological stageⅠandⅡ(P<0.01).The expression of miR-34 in patients with lymph node metastasis was significantly lower than that in patients without lymph node metastasis,and the expression of MDM2 and EPAS1 was significantly higher than that in patients without lymph node metastasis(P<0.01).The expression of miR-34 in patients with more than 1/2 myometrium infiltration was significantly lower than that in patients with less than 1/2 myometrium infiltration,and the expression of MDM2 and EPAS1 was significantly higher than that in patients with less

关 键 词：子宫内膜肿瘤 鼠双微基因2 内皮PAS1蛋白 淋巴转移 肿瘤浸润 

分 类 号：R737.33[医药卫生—肿瘤]