观赏鸡中禽白血病病毒分离鉴定及全基因测序分析  

Virus Isolation, Identification and Sequence Analysis of Avian Leukosis Viruses in Ornamental Chickens

作　　者：刘福林[1,2] 曹红 王亚楠[1] 武沛泽[1] 海伟 王宏钧 李三[1] 余德海[1] 陈福勇[1] 常建宇[1] LIU Fu-lin;CAO Hong;WANG Ya-nan;WU Pei-ze;HAI Wei;WANG Hong-jun;LI San;YU De-hai;CHEN Fu-yong;CHANG Jian-yu(College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;Sichuan Tianzow Breeding Technology Co.Ltd.,Chongqing 401100,China)

机构地区：[1]中国农业大学动物医学院,北京海淀100193 [2]四川天兆猪业股份有限公司,重庆渝北401100

出　　处：《中国兽医杂志》2020年第1期18-21,25,共5页Chinese Journal of Veterinary Medicine

基　　金：贵州省科技计划项目[黔科合NY字(2012)3028号]。

摘　　要：为了解观赏禽元宝鸡中禽白血病病毒(ALV)的流行特点,通过接种DF-1细胞、ELISA和PCR检测,从元宝鸡中分离并鉴定出1株禽白血病病毒,命名为BJ1401。经PCR扩增、测序获得BJ1401的全基因序列。序列比对分析发现,病毒株BJ1401基因全长7 503 bp,其中,gp85基因核苷酸序列与C亚群同源性最高(91.6%),gp37、LTR与E亚群相应核苷酸序列同源性最高,分别为98.2%、91.6%。进化分析显示,gp85与C亚群代表株Prague C亲缘关系最近,gp37、LTR与E亚群代表株ev-1和SD0501处在同一进化分支上。表明观赏性元宝鸡中分离的禽白血病病毒BJ1401可能是C亚型与E型禽白血病病毒的重组病毒。In order to investigate the gene characteristics of avian leukosis virus in ornamental chickens, one avian leukosis virus strain, named as BJ1401, was isolated and identified via DF1 cell culture, ELISA and PCR assay. One whole genome of ALV strain was amplified,cloned and sequenced from the genomic DNA of DF1+. Results showed that the full length cDNA of the BJ1401 strain was 7 503 bp.The gp85 nucleotide sequence in the isolate displayed a high identity(91.6%) with that of the gp85 gene in ALV-C. The gp37 and LTR nucleotide sequences had the highest similarities with those of ALV-E. Phylogenetic analysis of the nucleotide sequences showed that the gp85 nucleotide sequence in the isolate displayed a high genetic relationship with ALV-C representative strain Prague C. gp37 and LTR were in the same evolutionary branches with representative endogenous ALV strains ev1 and SD0501. The above results show that the isolate is a recombinant between ALV-C and ALV-E.

关 键 词：观赏鸡 禽白血病病毒 基因重组 

分 类 号：R730.[医药卫生—肿瘤]