狗枣猕猴桃多酚对60Coγ射线辐射损伤小鼠免疫系统保护作用的研究  

The Radioprotection of Actinidia Kolomikta Polyphenols against Organism Damage on the Immune System by 60Co γ-rays

作　　者：左丽丽 李昰奇 富校轶 高永欣 王振宇 王舒然 ZUO Li-li;LI Shi-qi;FU Xiao-yi;GAO Yong-xin;WANG Zhen-yu;WANG Shu-ran(School of Public Health,Jilin Medical College,Jilin 132013,China;School of Chemistry and Chemical Engineering,Harbin Institute of Technology,Harbin 150001,China)

机构地区：[1]吉林医药学院公共卫生学院,吉林吉林132013 [2]哈尔滨工业大学化工与化学学院,黑龙江哈尔滨150090

出　　处：《中国兽医杂志》2020年第1期107-112,115,共7页Chinese Journal of Veterinary Medicine

基　　金：吉林省科技厅优秀青年人才基金项目(20180520033JH);吉林省教育厅“十三五”科学技术项目(JJKH20180827KJ,JJKH20170411KJ);吉林医药学院大学生创新创业训练计划项目(201760)。

摘　　要：以狗枣猕猴桃为原料,研究分离纯化的狗枣猕猴桃多酚(AKP)的辐射防护作用。雄性昆明系小鼠随机分为6组:正常对照组、辐射模型组、利可君片组[1.4 mg/(kg·d)]、AKP低、中、高剂量组[分别为50、100、200 mg/(kg·d)],连续给药14 d,除正常对照组外所有小鼠采用^60Coγ射线进行全身照射,剂量率为0.792 Gy/min,辐射剂量为6.0 Gy,辐射后观察小鼠造血系统以及免疫系统的表达情况。结果显示,AKP能够保护小鼠的造血系统,提高辐射小鼠的脾脏指数、胸腺指数以及巨噬细胞的吞噬能力,并刺激IL-2、IL-4、IL-10、IFN-γ的产生,提高机体的免疫力,显著的降低了辐射对机体造成的伤害。表明AKP可一定程度地保护辐射对机体造成的损伤,同时为其进一步开发提供了科学依据。In this paper, selected Actinidia kolomikta was used as raw material to separate and purify Actinidia kolomikta polyphenols(AKP), and then investigate its radiation-protective effects. Male Kunming mice were divided into 6 groups randomly: control group, radiation model group, leucogen tablets group, low AKP group [50 mg/(kg·d)], medium AKP group [100 mg/(kg·d)], and high AKP group [200 mg/(kg·d)]. The interventions for AKP exposure continued for 14 consecutive days. Except the control group, all 5 groups were irradiated by ^60Co γ-rays. The dose rate was 0.792 Gy/min, and radiation dose was 6.0 Gy. After radiation, cytokine expressions of hemopoietic system and immune system were analyzed. The results indicated that AKP greatly protected hematopoietic system of mice, enhanced spleen index and thymus index and the phagocytosis of macrophage of radiating mice, and stimulated the production of interleukin IL-2, IL-4, IL-10, IFN-gamma. It also enhanced the body immunity, and significantly reduced the radiation damage to the body. These data suggest that AKP could greatly protect the body damage caused by radiation.

关 键 词：狗枣猕猴桃 多酚 氧化损伤 辐射防护 造血系统 免疫系统 

分 类 号：R285.5[医药卫生—中药学]