恒清Ⅰ号方对血管性痴呆大鼠学习记忆能力的保护作用研究  

Clinical Effects of HengqingⅠDecoction on Learning and Memory Ability of Rats with Vascular Dementia

作　　者：孟胜喜[1] 霍清萍[1] 王兵[1] 彭文波[1] 王宇新[1] 梁芳[1] 汪天湛[1] 张洪艳[1] 余忠海 彭学丰 李学敏[1] 曹健美[2] 李文涛[3] MENG Shengxi;HUO Qingping;WANG Bing;PENG Wenbo;WANG Yuxin;LIANG Fang;WANG Tianzhan;ZHANG Hongyan;YU Zhonghai;PENG Xuefeng;LI Xuemin;CAO Jianmei;LI Wentao(Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University,Shanghai 200233,China)

机构地区：[1]上海交通大学附属第六人民医院,上海200233 [2]上海中医药大学 [3]上海市中医医院

出　　处：《中西医结合心脑血管病杂志》2020年第12期1872-1875,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：上海市科委2019年度“科技创新行动计划”临床医学领域科技支撑项目(No.19401970600);上海市科委中医引导类项目(No.19401932500);上海市进一步加快中医药事业发展三年行动计划中医药重大临床研究[No.ZY(2018-2020)-CCCX-4010];上海交通大学医学院中西医结合创新基金项目(No.18zxy002);上海交通大学医学院教师培训发展项目(No.JFXM201909);上海交通大学2020年教育教学研究项目(No.JYJX200025)。

摘　　要：目的探讨恒清Ⅰ号方对血管性痴呆(VD)大鼠学习记忆能力的保护作用。方法将36只SD大鼠随机分为假手术组、模型组、奥拉西坦组及恒清Ⅰ号方组,各9只。以双侧颈总动脉结扎术制备VD大鼠模型,造模7 d后,恒清Ⅰ号方组大鼠给予中药恒清Ⅰ号方灌胃,奥拉西坦组给予奥拉西坦胶囊溶液灌胃,假手术组和模型组给予生理盐水灌胃,每日2次,灌胃干预4周、8周后,分别以Morris水迷宫实验评价各组大鼠学习记忆能力。结果与模型组比较,药物干预组(恒清Ⅰ号方组和奥拉西坦组)干预4周、干预8周大鼠逃避潜伏期(EL)均缩短,平台所在象限时间占比(PTSTQ)均增加,差异均有统计学意义(P<0.05或P<0.01);奥拉西坦组干预4周与8周比较,EL和PTSTQ无明显变化,差异均无统计学意义(P>0.05)。与恒清Ⅰ号方组干预4周比较,恒清Ⅰ号方组干预8周EL缩短,PTSTQ增加,差异均有统计学意义(P<0.05)。与奥拉西坦组干预8周比较,恒清Ⅰ号方组干预8周EL缩短,PTSTQ增加,差异均有统计学意义(P<0.05)。结论恒清Ⅰ号方可明显改善VD大鼠学习记忆能力,恒清Ⅰ号方干预8周优于干预4周,且优于奥拉西坦干预8周。Objective To investigate the effect of HengqingⅠdecoction on the learning and memory ability of rats with vascular dementia(VD).Methods Thirty-six SD rats were randomly divided into control group,model group,Oxiracetam group and HengqingⅠdecoction group,9 rats in each group.VD model was prepared by ligation of bilateral common carotid artery.Seven days after molding,HengqingⅠdecoction group were gavaged with HengqingⅠdecoction twice a day,Oxiracetam group were gavaged with Oxiracetam twice a day,the sham group and model group were both gavaged with normal saline twice a day.After intervention for 4 and 8 weeks,Morris water maze experiment was used to evaluate the learning and memory ability of rats in each group.Results After intervention for 4 and 8 weeks,compared with model group,escape latencies(EL)of the treatment group(HengqingⅠdecoction group and Oxiracetam group)were shortened and percentages of time spent in the target quadrant(PTSTQ)was prolonged(P<0.05 or P<0.01).There were no significant statistic difference in EL and PTSTQ of Oxiracetam group between Oxiracetam 4 weeks intervention and 8 weeks intervention(P>0.05).Compared to HengqingⅠdecoction group for 4 weeks intervention,EL of HengqingⅠdecoction group for 8 weeks intervention was shorter,PTSTQ was longer(P<0.05).Compared to Oxiracetam group for 8 weeks intervention,EL of HengqingⅠdecoction group for 8 weeks intervention was shorter,PTSTQ was longer(P<0.05).Conclusion HengqingⅠdecoction might obviously improve the vascular dementia learning and memory ability of rats.The effect of HengqingⅠdecoction intervention for 8 weeks was not only superior to the effect of HengqingⅠdecoction intervention for 4 weeks,but also superior to the effect of Oxiracetam intervention for 8 weeks.

关 键 词：血管性痴呆 恒清Ⅰ号方 水迷宫 学习记忆 奥拉西坦 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学] R289.5[医药卫生—临床医学]