野地瓜茎正丁醇萃取物中主要抗氧化活性成分的筛选  

Screening of main antioxidant components in n-butanolextract from the stems of Ficus tikoua Bur

作　　者：刘永玲 叶思帆 杨怡 方丽红 赵治兵 谢国芳 LIU Yong-ling;YE Si-fan;YANG Yi;FANG Li-hong;ZHAO Zhi-bing;XIE Guo-fang(Food and Pharmaceutical Engineering Institute,Guiyang University,Guiyang,Guizhou 550005,China;Guizhou Engineering Research Center for Fruit Processing,Guiyang,Guizhou 550005,China;Hunan University,Changsha,Hunan 410082,China)

机构地区：[1]贵阳学院食品与制药工程学院,贵州贵阳550005 [2]贵州省果品加工工程技术研究中心,贵州贵阳550005 [3]湖南大学,湖南长沙410082

出　　处：《食品与机械》2020年第6期176-181,共6页Food and Machinery

基　　金：贵州省教育厅青年科技人才成长项目(编号:黔教合KY字[2017]244);2019年度贵阳市科学技术局—贵阳学院科技专项资金项目(编号:GYU-KYZ[2019~2020]平台PT16-06号);贵州省教育厅高等学校拔尖人才计划(编号:黔教合KY字[2019]066号)。

摘　　要：探清野地瓜茎不同极性萃取物的抗氧化活性,筛选并鉴定抗氧化活性较高萃取物的主要化学成分。通过系统溶剂法对野地瓜茎提取物进行萃取,获得石油醚、二氯甲烷、乙酸乙酯、正丁醇相提取物及剩余上层被萃取水相提取物;采用1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力、2,2’-联氮基-双-(3-乙基苯并噻唑-6-磺酸)二铵盐(ABTS)自由基清除能力和总还原力(TRPA)试验综合评价其不同极性萃取物的抗氧化活性;结合在线高效液相色谱—质谱—二苯基三硝基苯肼(HPLC-DAD-ESI/MS n-DPPH)快速筛选并鉴定活性较高萃取物中的抗氧化活性成分。结果表明:正丁醇萃取物具有较强的抗氧化活性(P<0.05),随着质量浓度的增大呈明显的剂量依赖效应,正丁醇萃取物对DPPH自由基、ABTS自由基清除能力的半数抑制浓度(IC 50)分别为(23.28±0.21),(76.30±1.13)μg/mL。经液相色谱、质谱、文献报道和对照品的综合分析,从野地瓜茎正丁醇萃取物中筛选的3种抗氧化活性化合物为5-O-咖啡酰奎宁酸、3-O-咖啡酰奎宁酸和4-O-咖啡酰奎宁酸。To investigate the antioxidant activity of different polar extracts from the stems of Ficus tikoua Bur.(FTB),the main chemical components of the highly active polar extracts were screened and identified.Petroleum ether,dichloromethane,ethyl acetate,n-butanol extract and water extract of the remaining upper layer were obtained by systematic solvent method.The antioxidant activities of different polar extracts were evaluated by the scavenging ability of 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2’-azino-bis-(3-ethylbenzothiazole-6-sulfonic acid)diammonium salt(ABTS)and total reducing ability(TRPA).HPLC-DAD-ESI/MS n-DPPH was used to quickly screen and identify the antioxidant characteristic components of higher polar parts from FTB stems.The results showed that the n-butanol extract had strong antioxidant activity(P<0.05).With the concentration increasing,there is a significant dose-dependent effect.The IC 50 of n-butanol extract to DPPH and ABTS were(23.28±0.21)μg/mL and(76.30±1.13)μg/mL,respectively.Three compounds were identified to be 5-O-caffeoylquinic acid,3-O-caffeoylquinic acid and 4-O-caffeoylquinic acid through comprehensive analysis of HPLC spectra,MS spectra,literature data and reference standards.

关 键 词：野地瓜茎 抗氧化活性 活性成分 高效液相色谱—质谱—二苯基三硝基苯肼 

分 类 号：TQ28[化学工程—有机化工]