缺氧诱导因子-1α在人与小鼠口腔癌形成过程中的表达分析  

Expression analysis of hypoxia inducible factor-1α in the formation of oral cancer in humans and mice

作　　者：陈凤强 刘正武[1] 于大海[2] 李晶[2] 王洁[3] CHEN Fengqiang;LIU Zhengwu;YU Dahai;LI Jing;WANG Jie(Department of Stomatology,the Second Nanning People’s Hospital,Nanning 530031,Guangxi,China;Department of Stomatology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China;Department of Science and Education,the Second Nanning People’s Hospital,Nanning 530031,Guangxi,China)

机构地区：[1]南宁市第二人民医院口腔科,广西南宁市530031 [2]广西医科大学第一附属医院口腔科,广西南宁市530021 [3]南宁市第二人民医院科教部,广西南宁市530031

出　　处：《微创医学》2020年第3期283-287,共5页Journal of Minimally Invasive Medicine

基　　金：广西教育厅科研项目(编号:桂教科研2003)。

摘　　要：目的对比分析缺氧诱导因子-1α(HIF-1α)在人与BALB/c小鼠正常口腔黏膜上皮、异常增生、高分化鳞癌及颌下淋巴结转移灶中的表达情况。方法通过4-硝基喹啉-1-氧化物(4-NQO)饮水法造模,培养口腔黏膜上皮产生异常增生、鳞癌及其颌下淋巴结转移的BALB/c小鼠20只,另收集人正常口腔黏膜上皮、异常增生、高分化鳞癌及其颌下淋巴结转移灶标本各20份,用免疫组织化学方法检测各标本HIF-1α的表达情况,比较人和BALB/c小鼠中正常口腔黏膜上皮、异常增生、高分化鳞癌及颌下淋巴结转移灶中HIF-1α的表达差异。结果人和小鼠HIF-1α阳性表达率均呈由正常口腔黏膜上皮、异常增生到口腔高分化鳞癌的上升趋势,但颌下淋巴结转移灶表达较原发灶明显降低。HIF-1α阳性表达率在正常口腔黏膜上皮、异常增生和口腔高分化鳞癌中的差异均有统计学意义(均P<0.05)。结论 HIF-1α在人与小鼠正常口腔黏膜上皮、异常增生、高分化鳞癌及其颌下转移淋巴结相应阶段的表达相似。Objective To compare and analyze the expressions of hypoxia inducible factor-1α(HIF-1α) between normal oral mucosa epithelium, abnormal hyperplasia, high-differentiated squamous cell carcinoma, and submaxillary lymph node metastasis in humans and BALB/c mice. Methods Modeling according to the method of 4-nitroquinoline-1-oxide(4-NQO) water drink, 20 BALB/c mice with abnormal hyperplasia occurring in the oral mucosa epithelium, squamous carcinoma, and submaxillary lymph node metastasis were cultured. In addition, 20 samples each from humans with normal oral mucosa epithelium, abnormal hyperplasia, high-differentiated squamous cell carcinoma, and submaxillary lymph node metastasis were collected, using the immunohistochemistry to detect expressions of HIF-1α in various samples, and to compare the expression differences of HIF-1α in normal oral mucosa epithelium, abnormal hyperplasia, high-differentiated squamous cell carcinoma, and submaxillary lymph node metastasis between humans and BALB/c mice. Results The positive rate of HIF-1α in humans and mice all presented an ascending order following normal oral mucosa epithelium, abnormal hyperplasia, and oral high-differentiated squamous cell carcinoma;however, the expression of submaxillary lymph node metastasis lesions decreased significantly as compared with original lesions. There were statistically significant differences in HIF-1α positive rates of expressions in normal oral mucosa epithelium, abnormal hyperplasia, and high-differentiated squamous cell carcinoma(all P<0.05). Conclusion Expressions of the corresponding stages in normal oral mucosa epithelium, abnormal hyperplasia, high-differentiated squamous cell carcinoma, and submaxillary lymph node metastasis are similar in humans and mice.

关 键 词：缺氧诱导因子-1Α 口腔黏膜上皮 异常增生 高分化鳞癌 

分 类 号：R739.85[医药卫生—肿瘤]