UPLC-MS/MS法同时测定六味调更片中4种成分  

Simultaneous determination of four constituents in Liuwei Tiaogeng Tablets by UPLC⁃MS/MS

作　　者：余琰[1,2] 郭玫 邵晶[1,3] 李娟[1] 武权生 YU Yan;GUO Mei;SHAO Jing;LI Juan;WU Quan⁃sheng(Gansu University of Traditional Chinese Medicine,Lanzhou 730101,China;Gansu Provincial Key Laboratory for Traditional Chinese Medicine Quality and Standard,Lanzhou 730000,China;Gansu Provincial Colleges’Key Laboratory for Chemistry and Quality of Traditional Chinese Medicine&Tibetan Medicine,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730101 [2]甘肃省中药质量与标准研究重点实验室,甘肃兰州730000 [3]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000

出　　处：《中成药》2020年第7期1718-1720,共3页Chinese Traditional Patent Medicine

基　　金：2018年甘肃省中医药管理局科研课题(GZK⁃2018⁃7);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金(ZZY⁃2016⁃03);甘肃省中药质量与标准研究重点实验室培育基地开放基金(ZYZL17⁃001)。

摘　　要：目的建立UPLC-MS/MS法同时测定六味调更片(黄芪、当归、女贞子等)中4种成分的含有量。方法该药物甲醇提取液的分析采用CAPCELL PAK C_(18) MGⅢ色谱柱(2.0 mm×150 mm,5μm);流动相0.1%甲酸-乙腈,梯度洗脱;体积流量0.3 mL/min;柱温40℃。采用电喷雾电离源,正负离子全扫描模式。结果毛蕊异黄酮-7-O-β-D-葡萄糖苷、阿魏酸、特女贞苷、黄芪甲苷分别在0.985 8~98.576、0.407 7~40.768、4.823 6~482.361、2.097 9~209.789 ng/mL范围内的线性关系良好(r>0.999 0),平均加样回收率分别为98.5%、101.5%、99.4%、98.4%,RSD分别为2.1%、1.8%、1.1%、1.9%。结论该方法简便、快速、准确,可用于六味调更片的质量控制。AIM To establish a UPLC⁃MS/MS method for the simultaneous content determination of four constituents in Liuwei Tiaogeng Tablets(Astragali Radix,Angelicae sinensis Radix,Ligustri lucidi Fructus,etc.).METHODS The analysis of methanol extract of this drug was performed on a 40℃thermostatic CAPCELL PAK C18 MGⅢcolumn(2.0 mm×150 mm,5μm),with the mobile phase comprising of 0.1%formic acid⁃acetonitrile flowing at 0.3 mL/min in a gradient elution manner.Electrospray ionization source was adopted in positive/negative ion full scanning mode.RESULTS Calycosin⁃7⁃O⁃β⁃D⁃glucoside,ferulic acid,specnuezhenide,astragaloside A showed good linear relationships within the ranges of 0.9858-98.576,0.4077-40.768,4.8236-482.361,2.0979-209.789 ng/mL(r>0.9990).whose average recoveries were 98.5%,101.5%,99.4%,98.4%with the RSDs of 2.1%,1.8%,1.1%,1.9%,respectively.CONCLUSION This simple,rapid and accurate method can be used for the quality control of Liuwei Tiaogeng Tablets.

关 键 词：六味调更片 毛蕊异黄酮-7-O-β-D-葡萄糖苷 阿魏酸 特女贞苷 黄芪甲苷 UPLC-MS/MS 

分 类 号：R927.2[医药卫生—药学]