固相萃取-气相色谱-三重四级杆质谱法测定蔬菜中五氯硝基苯和百菌清残留量  

Determination of pentachloronitrobenzene and chlorothalonil residues in vegetables by solid phase extraction-gas chromatography triple quadrupole mass spectrometry

作　　者：曹忠波[1] 张媛媛[1] 刘晓晶[1] 华正罡[1] CAO Zhong-Bo;ZHANG Yuan-Yuan;LIU Xiao-Jing;HUA Zheng-Gang(Center for Disease Control and Prevention of Liaoning,Shengyang 110005,China)

机构地区：[1]辽宁省疾病预防控制中心,沈阳110005

出　　处：《食品安全质量检测学报》2020年第12期4132-4136,共5页Journal of Food Safety and Quality

摘　　要：目的建立固相萃取-气相色谱-三重四级杆质谱法测定蔬菜中五氯硝基苯和百菌清残留量的检测方法。方法样品采用正己烷溶剂提取,串联Florisil-Carb固相萃取小柱净化,采用多重反应监测(multiple reaction monitoring,MRM)模式检测,内标法进行定量。结果五氯硝基苯和百菌清的平均回收率分别在78.7%~91.6%、80.2%~90.8%之间;检出限(S/N=3)为0.005 mg/kg和0.004 mg/kg。结论该方法具有灵敏度高、分离效果好、重复性好的特点,适用于大批量蔬菜样品中五氯硝基苯和百菌清杀菌剂残留检测的要求。Objective To establish a method for the determination of quintozene and chlorothalonil residues in vegetables by solid phase extraction-gas chromatography-triple quadrupole mass spectrometry.Methods The samples were extracted with N-hexane solvent,purified with Florisil-carb solid phase extraction column in series,detected by multiple reaction monitoring(MRM)mode,and quantified by internal standard method.Results The average recoveries of quintozene and chlorothalonil were 78.7%–91.6%and 80.2%–90.8%,and the detection limits(S/N=3)were 0.005 mg/kg and 0.004 mg/kg,respectively.Conclusions This method has the characteristics of high sensitivity,good separation effect and good repeatability.It is suitable for the detection of pentachloronitrobenzene and chlorothalonil residues in a large number of vegetable samples.

关 键 词：固相萃取 五氯硝基苯和百菌清 气相色谱-三重四级杆质谱联用仪 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]