阴地蕨粗多糖对细菌和肿瘤细胞增殖的抑制作用  

Inhibition effects of crude polysaccharides from Botrychium ternatum on bacteria and proliferation of tumor cells

作　　者：周施雨 钱志萍 曹东东 於俊杰 游蕤翔 戴锡玲[1] ZHOU Shiyu;QIAN Zhiping;CAO Dongdong;YU Junjie;YOU Ruixiang;DAI Xiling(Shanghai Normal University:a.College of Life Sciences,Shanghai 200234,China;Shanghai Normal University:School of Environmental and Geographical Sciences,Shanghai 200234,China;Shanghai General Hospital,Shanghai 200080,China)

机构地区：[1]上海师范大学生命科学学院,上海200234 [2]上海师范大学环境与地理科学学院,上海200234 [3]上海市第一人民医院,上海200080

出　　处：《植物资源与环境学报》2020年第4期69-71,共3页Journal of Plant Resources and Environment

基　　金：上海市绿化和市容管理局科学技术攻关项目(G152430);上海植物种质资源工程技术研究中心项目(17DZ2252700)。

摘　　要：近年来,蕨类植物的有效成分研究备受人们关注,药用蕨类植物含有黄酮类、酚酸类、萜类、生物碱和多糖等化学成分[1];其多糖除了参与构成细胞壁和储藏营养外,还具有提高免疫力、抗菌和抗癌的功效[2-3]。阴地蕨〔Botrychium ternatum(Thunb.)Sw.〕隶属于阴地蕨科(Botrychiaceae)阴地蕨属(Botrychium Sw.),为传统药用植物,全草入药,具有清热解毒、平肝息风、止咳、止血和明目去翳的功效[4-5]。Crude polysaccharides were extracted from the whole plant of Botrychium ternatum(Thunb.)Sw.by using water extraction and ethanol precipitation method,and their inhibition effects on bacteria and proliferation of tumor cells were analyzed.The results show that the yield of crude polysaccharides from the whole plant of B.ternatum is 7.05%,and polysaccharides content in crude polysaccharides is 20.60%.Crude polysaccharides from B.ternatum show a certain inhibition effect on Escherichia coli and Staphyloccocus aureus,and that on E.coli is stronger than that on S.aureus,and the minimum inhibitory concentration is 0.206 and 0.309 mg·mL-1,respectively;crude polysaccharides from B.ternatum also show an inhibition effect on proliferation of A-549 and Hela cells,and that on proliferation of Hela cell is stronger than that on A-549 cell,the semi-inhibitory concentration is 138.90 and 109.23μg·mL-1,respectively.Inhibition effects of crude polysaccharides from B.ternatum on bacteria and proliferation of tumor cells are all evidently smaller than those of several positive control,but show a tendency to gradually increase with the increase of mass concentration,and a certain concentration dependence.

关 键 词：阴地蕨 粗多糖 抑菌作用 肿瘤细胞增殖 

分 类 号：Q949.95[生物学—植物学] R285.5[医药卫生—中药学]