转华山新麦草果聚糖合成酶基因对烟草相关生理指标的影响  

Effects of transfer of fructan synthase gene of Psathyrostachys huashanensis on physiological indexes of tobacco

作　　者：陈真真 周国勤 陈新宏[2] 谢旭东 陈金平 CHEN Zhen-zhen;ZHOU Guo-qin;CHEN Xin-hong;XIE Xu-dong;CHEN Jin-ping(Xinyang Academy of Agricultural Sciences,Xinyang 464000,Henan,China;College of Agriculture,Northwest University of Agriculture and Forestry,Yangling 712100,Shaanxi,China)

机构地区：[1]信阳市农业科学院,河南信阳464000 [2]西北农林科技大学农学院,陕西杨凌712100

出　　处：《湖北农业科学》2020年第9期95-98,共4页Hubei Agricultural Sciences

基　　金：国家科技支撑计划项目(2015BAD26B01);2018年河南省重大科技专项(181100110200);河南省小麦产业技术体系支持项目(Z2010-01-01);国家小麦产业技术体系支持项目(CARS-03)。

摘　　要：以转华山新麦草果聚糖合成酶基因(HS-6-SFT)烟草和非转基因烟草W38为材料,在4℃低温下对其胁迫8 h,分析转HS-6-SFT基因烟草和非转基因烟草的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性和可溶性蛋白质含量的变化。结果表明,在低温胁迫下烟草叶片中的SOD活性、CAT活性、POD活性和可溶性蛋白质含量均有所增加,且增加的幅度要大于常温处理,并且在转基因材料和对照之间存在极显著差异(P<0.01)。转HS-6-SFT基因增强了烟草的抗逆性。HS-6-SFT transgenic tobacco and non-transgenic tobacco W38 were used as materials under low temperature stress at4 ℃ for 8 h. The differences of soluble protein,superoxide dismutase(SOD),catalase CAT,peroxidase(POD)between transgenic tobacco and non-transgenic tobacco were analyzed. The results showed that the soluble protein content,SOD activity,CAT activity and POD activity in tobacco leaves increased,and the increase at 4 ℃ was larger than that at room temperature. There was a very significant difference between the transgenic materials and the control(P<0.01). The stress resistance of transgenic tobacco with HS-6-SFT gene is enhanced.

关 键 词：转基因烟草 超氧化物歧化酶(SOD) 过氧化氢酶(CAT) 过氧化物酶(POD) 可溶性蛋白质 低温胁迫 

分 类 号：S572[农业科学—烟草工业]