改性蛭石吸附-包埋法固定化脂肪酶的研究  

Lipase immobilization by modified vermiculite adsorption-embedding method

作　　者：黄静[1] 梁密 HUANG Jing;LIANG Mi(College of Science,Liaoning Technical University,Fuxin 123000,China;College of Material,Liaoning Technical University,Fuxin 123000,China)

机构地区：[1]辽宁工程技术大学理学院,辽宁阜新123000 [2]辽宁工程技术大学材料学院,辽宁阜新123000

出　　处：《食品与发酵工业》2020年第14期103-107,共5页Food and Fermentation Industries

基　　金：2017年辽宁省教育厅青年项目(理)(LJ2017QL030)。

摘　　要：为了使脂肪酶在固定化的过程中酶活力损失小的同时提高固定化脂肪酶的稳定性,对325目蛭石和1 000目蛭石进行酸改性,选取比表面积大、孔径结构特征丰富的改性后蛭石为载体,采用吸附-包埋法固定化脂肪酶,研究改性蛭石作为载体固定化脂肪酶的最优化条件及固定化脂肪酶的催化性能。分析表明,1 000目蛭石经过改性后比表面积比较大、孔径结构较丰富。脂肪酶的最优固定化工艺为:脂肪酶与载体的质量比为1∶1,吸附温度为25℃,吸附时间为3 h,海藻酸钠的质量浓度为20 g/L,明胶的质量浓度为5 g/L,Ca Cl2溶液的浓度为0. 04 mol/L,在此条件下固定化脂肪酶的酶活力回收率可达到82. 77%;固定化脂肪酶经过7次重复回收利用后,酶活力仍可保留原来的85. 33%,说明其重复使用稳定性较好。To reduce the loss of lipase activity during immobilization and improve the stability of immobilized lipase,325-mesh and 1 000-mesh vermiculites were modified by acid. The modified vermiculite with large specific surface area and rich pore structure was selected as the carrier,and lipase was immobilized by the adsorption-embedding method. The optimal conditions for immobilizing lipase with modified vermiculite as the carrier and the catalytic activity of immobilized lipase were investigated. The results showed that the specific surface area was relatively large and the pore structure of vermiculite was relatively rich when 1 000-mesh vermiculite was used. The optimal immobilization conditions of lipase were as follows: the mass ratio of lipase to carrier was 1∶ 1;the adsorption temperature was 25 ℃;the adsorption time was 3 h;the concentration of sodium alginate was 20 g/L;and the concentration of gelatin was 5 g/L. Calcium chloride at 0. 04 mol/L enhanced the recovery rate of the immobilized lipase to 87. 27%;after 7 rounds of recycling,the enzymatic activity was 85. 33% of that of the original,which indicated good stability of the enzyme.

关 键 词：改性蛭石 脂肪酶 固定化 催化特性 吸附 包埋 

分 类 号：Q814.2[生物学—生物工程]