罗布麻叶的HPLC指纹图谱及抗氧化活性谱效关系研究  

Relationship between HPLC fingerprints and antioxidant activities of Folium Apocyni veneti

作　　者：徐硕[1] 金鹏飞[1] 徐文峰[1] 邝咏梅[1] 姜文清[1] XU Shuo;JIN Pengfei;XU Wenfeng;KUANG Yongmei;JIANG Wenqing(Department of Pharmaceutical Science,Beijing Hospital,National Center of Gerontology,Beijing Key Laboratory of Drug Clinical Risk and Personalized Medication Evaluation,Beijing 100730,China)

机构地区：[1]北京医院药学部,国家老年医学中心,药物临床风险与个体化应用评价北京市重点实验室,北京100730

出　　处：《西北药学杂志》2020年第4期475-480,共6页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金青年科学基金项目(编号:81303216)。

摘　　要：目的建立罗布麻叶的指纹图谱并考察其体外抗氧化活性的谱效关系,为揭示其抗氧化活性物质基础提供参考。方法采用HPLC法建立20批罗布麻叶的指纹图谱,不同产地罗布麻叶体外抗氧化活性的测试采用1,1-二苯基-2-三硝基苯肼(DPPH·)自由基清除法,利用灰色关联度分析(GRA)法和双变量相关性分析(BCA)法对谱效关系进行探讨。结果建立了20批罗布麻叶HPLC指纹图谱,确定了18个共有峰,相似度在0.883~0.989范围内。运用对照品比对法鉴定了其中7个峰:X2为绿原酸,X7为芦丁,X8为金丝桃苷,X9为异槲皮苷,X12为紫云英苷,X17为槲皮素,X18为山柰酚。对样品的指纹图谱进行聚类分析,结果显示可聚为4类。20批罗布麻叶均有不同程度的清除自由基活性。GRA法分析结果显示,18个共有峰与清除自由基的关联度在0.6917~0.9593范围内,大小依次为X2>X9>X8>X17>X18>X12>X11>X7>X13>X15>X14>X1>X10>X3>X5>X6>X16>X4。BCA法分析结果显示,2号峰(绿原酸)、8号峰(金丝桃苷)、9号峰(异槲皮苷)、17号峰(槲皮素)和18号峰(山柰酚)的含量变化与DPPH·自由基清除活性大小密切相关。结论市售不同产地罗布麻叶的化学成分基本一致,但不同产地的药材质量有一定差异,罗布麻叶抗氧化作用是多成分共同起效的结果。通过建立HPLC指纹图谱及分析谱效关系探寻罗布麻叶抗氧化物质基础,可为该药材的质量评价提供参考。Objective To establish the fingerprints of Folium Apocyni veneti,investigate the spectrum-effect relationship between HPLC fingerprints and antioxidant activities in vitro,and provide reference for revealing the material basis for its antioxidant activity.Methods HPLC was conducted to establish the fingerprints of 20 batches of Folium Apocyni veneti.The 1,1-diphenyl-2-trinitrobenzene hydrazine hydrazine(DPPH·)free radical scavenging method was used to determine the antioxidant activity in vitro of Folium Apocyni veneti from different regions.The methods of grey relational analysis(GRA)and bivariate correlation analysis(BCA)were adopted to explore the spectrum-effect relationship.Results The HPLC fingerprints of 20 batches of Folium Apocyni veneti were established,and 18 common peaks were identified with the similarities between 0.883-0.989.7 peaks among common peaks were identified by comparing with reference substances.X2,X7,X8,X9,X12,X17 and X18were identified as chlorogenic acid,rutin,hyperoside,isoquercitrin,astragalin,quercetin and kaempferol,respectively.Cluster analysis of fingerprints of the samples showed that they can be grouped into 4 categories.All of the samples had different degrees of free radical scavenging activity.The result of GRA demonstrated that correlations between 18 common peaks and free radical scavenging were in the range of 0.6917-0.9593,with the order of X2,X9,X8,X17,X18,X12,X11,X7,X13,X15,X14,X1,X10,X3,X5,X6,X16 and X4from high to low.The results of BCA showed that the contents of peak 2(chlorogenic acid),peak 8(hyperoside),peak 9(isoquercitrin),peak 17(quercetin)and peak 18(kaempferol)were closely related to DPPH·free radical scavenging activity.Conclusion The chemical constituents of commercially available Folium Apocyni veneti from different regions are basically the same,but there are some differences in quality between those from different regions.The antioxidant effect of Folium Apocyni veneti is the result of multi-components acting together.Exploring the antioxidant materia

关 键 词：罗布麻叶 指纹图谱 抗氧化活性 谱效关系 HPLC法 

分 类 号：R284[医药卫生—中药学]