PPAR-α激动剂非诺贝特抑制前列腺癌PC-3细胞生长和诱导凋亡的作用研究  

PPAR-αAgonist Fenofibrate Inhibits the Growth and Induces Apoptosis of the Prostate Cancer Cell Line PC-3

作　　者：陈鹏飞 于蕾 肖云峰[3] 岳根全[4] CHEN Peng-fei;YU Lei;XIAO Yun-feng;YUE Gen-quan(Department of Pharmacy,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,China;Department of Pharmacy,Inner Mongolia Hospital of Traditional Chinese Medicine,Hohhot 010020,China;The Center for New Drug Safety Evaluation and Research of Inner Mongolia Medical University,Hohhot 010110,China;Department of Urology,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院药剂科,内蒙古呼和浩特010050 [2]内蒙古自治区中医医院药剂科,内蒙古呼和浩特010020 [3]内蒙古医科大学新药安全评价研究中心,内蒙古呼和浩特010110 [4]内蒙古医科大学附属医院泌尿外科,内蒙古呼和浩特010050

出　　处：《内蒙古师范大学学报（自然科学汉文版）》2020年第4期298-302,308,共6页Journal of Inner Mongolia Normal University(Natural Science Edition)

基　　金：内蒙古自然科学基金资助项目(2015MS08115);内蒙古医科大学附属医院重大科研资助项目(NYFYZD2014011);内蒙古自治区卫生计生科研计划资助项目(201701066)。

摘　　要：探讨过氧化物酶体增殖因子激活受体α(PPAR-α)激动剂非诺贝特对前列腺癌PC-3细胞生长的抑制和诱导凋亡的作用。分别用0、25、50、75、100μmol/L浓度的非诺贝特作用于PC-3细胞,经过24 h、48 h、72 h后,分别采用MTT、Western blot检测不同组别PC-3细胞的生长或凋亡情况。MTT结果显示:非诺贝特对PC-3细胞的增值存在显著的抑制影响,并且抑制效果和所加入的溶液浓度存在正相关性;流式细胞结果表明:非诺贝特经过24 h、48 h及72 h处理后,测定得到的凋亡细胞总百分比分别为8.41%±2.05%、16.77%±3.16%和23.65%±4.43%,较对照组4.65%±1.12%明显升高。Western blot的检测结果显示:50μmol/L非诺贝特处理PC-3细胞,48 h后其凋亡因子Caspase 3和AIF的表达均有明显的增加。据此,非诺贝特能够显著激活前列腺癌PC-3细胞凋亡因子Caspase 3和AIF的表达,实现诱导细胞凋亡,从而表现出对PC-3细胞增殖的抑制作用。To study the inhibitory effect of peroxisome proliferator-activated receptor alpha agonist fenofibrate on the growth and apoptosis of androgen-independent prostate cancer PC-3 cells and the possible mechanism,the PC-3 cell was treated with 0,25,50,75 and 100μmol/L of fenofibrate to detected its proliferation and apoptosis by MTT assay and flow cytometry at 24 h,48 h and 72 h and expression of apoptosis marker Caspase 3 and apoptosis inducing factor(AIF)by Western blotting.The results of MTT showed that fenofibrate significantly inhibited the growth of PC-3 cells in a time and dose dependent manner and the flow cytometry analysis indicated that the total percentage of apoptotic cells treated with fenofibrate at 24,48 and 72 h were 8.41%±2.05%,16.77%±3.16%and 23.65%±4.43%respectively,significantly higher than 4.65%±1.12%of the control group.Western blotting revealed that the expression of Caspase 3 and AIF was significantly increased with fenofibrate treatment for 48 h.The research demonstrated that fenofibrate inhibited the growth of PC-3 cells mainly by inducing apoptosis through activating Caspase 3 and AIF.

关 键 词：前列腺癌 非诺贝特 细胞增殖 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]