Knockdown of Nav1_5 inhibits cell proliferation,migration and invasion via Wnt/β-catenin signaling pathway in oral squamous cell carcinoma  

作　　者：Xiaoli Xu Yongzheng Dai Linfei Feng Hongli Zhang Yukun Hu Le Xu Xinwei Zhu Yong Jiang 

机构地区：[1]College and Hospital of Stom atology,Anhui Medical University,Key Laboratory of Oral Diseases Research of Anhui Province,Hefei 230032,China [2]Hefei School of Stomatology,Anhui M edical University,Hefei 230001,China [3]Departm ent of General Dentistry,Hefei Stom atological Hospital,Hefei 230001,China [4]Departm ent of Oral and Maxillofacial Surgery,the First A ffiliated Hospital of Anhui Medical University,Hefei 230022,China [5]Departm ent of Stomatology,the Fourth Affiliated Hospital of Anhui Medical University,Hefei 230000,China [6]Binhu Clinical Division,Anhui Stom atology Hospital Affiliated to Anhui M edical University,Hefei 230601,China

出　　处：《Acta Biochimica et Biophysica Sinica》2020年第5期527-535,共9页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the 2017 Anhui Public Welfare Technology Application Research Contact Plan(No.1704(0804020),the Anhui Provincial Natural Science Foundation of China(No.1508085MH187),and the Anhui Medical University Research Fund Project(No.2018xkj063).

摘　　要：Oral squamous cell carcinoma(OSCC)is a common type of malignant oral cancer that has a high recurrence rate.Voltage-gated sodium channel Nav1.5 was reported to be highly up-regulated in various types of cancers.However,the regulatory mechanism of Nav1.5 in cancers including OSCC still remains elusive.In this study,Nav1.5 was found to be highly expressed in OSCC tissues and cells.Through the analysis of clinical characteristics of patients,we found that the expression level of Nav1.5 was closely related to neutrophil-to-lymphocyte ratio,platelet-to-lymphocyte ratio,tumor-node-metastasis stage,and lymph node metastasis.Moreover,we found that Nav1.5 mainly located on the cell membrane as well as cytoplasm and knockdown of Nav1.5 promoted cell apoptosis and decreased proliferation in OSCC.Transwell assay results showed that knockdown of Nav1.5 effectively suppressed the migration and invasion in OSCC.In addition,knockdown of Nav1.5 was found to inhibit the protein and mRNA expression levels ofβ-catenin,cyclin D1,and C-Myc in the Wnt/βcatenin signaling pathway.In summary,these results indicated that Nav1.5 may be involved in the progression of OSCC through the Wnt/βcatenin signaling pathway.

关 键 词：oral squamous cell carcinoma(OSCC).Nav1.5 WntB-catenin signaling pathway 

分 类 号：R739.8[医药卫生—肿瘤]