CD34^+细胞移植对大鼠放射性脑损伤的影响及其与星形胶质细胞活性的关系  

Effects of CD34+cell transplantation on radiation-induced brain injury and relationship with activity of astrocytes in rats

作　　者：孙熠[1] 王海云[1] 赵茗姝 金学隆 华伟 Sun Yi;Wang Haiyun;Zhao Mingshu;Jin Xuelong;Hua Wei(Department of Anesthesiology,the Third Central Clinical College of Tianjin Medical University,the Third Central Hospital of Tianjin,Tianjin 300170,China;Department of Physiology and Pathophysiology,Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]天津医科大学三中心临床学院天津市第三中心医院麻醉科,300170 [2]天津医科大学基础医学院生理学与病理生理学系,300070

出　　处：《中华麻醉学杂志》2020年第2期182-185,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价CD34+细胞移植对大鼠放射性脑损伤的影响及其与星形胶质细胞活性的关系。方法健康成年雄性SD大鼠108只,体重210~230 g,采用随机数字表法分为3组(n=36):对照组(C组)、放射性脑损伤组(RIBI组)和CD34+组。采用CT照射建立放射性脑损伤模型。另取6只大鼠,采用流式细胞术分离提取CD34+细胞,并进行BrdU标记。CD34+组于建模后第7天进行CD34+细胞移植。C组和RIBI组于建模后7、14和28 d时,CD34+组于建模后14和28 d时,取脑组织,测定伊文思蓝(EB)渗透比值,采用免疫组化法测定胶质纤维酸性蛋白(GFAP)表达水平。结果与C组比较,RIBI组建模后EB渗透比值升高,GFAP表达上调(P<0.05),CD34+组建模后EB渗透比值差异无统计学意义(P>0.05)。与RIBI组比较,CD34+组建模EB渗透比值降低,GFAP表达下调(P<0.05)。结论 CD34+细胞移植可减轻大鼠放射性脑损伤,机制可能与抑制星形胶质细胞活性有关。Objective To evaluate the effects of CD34+cell transplantation on radiation-induced brain injury(RIBI)and the relationship with the activity of astrocytes in rats.Methods Healthy adult male Sprague-Dawley rats,weighing 210-230 g,were divided into 3 groups(n=36 each)using a random number table method:control group(C group),RIBI group,and CD34+group.RIBI model was established by computed tomography(CT)scanning in anesthetized rats.Another 6 rats were selected,and CD34+cells were eluted by flow cytometry and labeled with BrdU.CD34+cells were transplanted at day 7 after establishing the model.Brain tissues were obtained at 7,14 and 28 days after establishing the model in C and RIBI groups and at 14 and 28 days after establishing the model in CD34+group for determination of Evans blue(EB)extravasation ratio and expression of GFAP(by immuno-histochemistry).Results Compared with group C,the EB extravasation ratio was significantly increased after establishing the model,and the expression of GFAP was up-regulated in group RIBI(P<0.05),and no significant change was found in EB extravasation ratio after establishing the model in group CD34+(P>0.05).Compared with group RIBI,the EB extravasation ratio was significantly decreased after establishing the model,and the expression of GFAP was down-regulated in group CD34+(P<0.05).Conclusion CD34+cell transplantation can reduce RIBI,and the mechanism may be related to inhibiting the activity of astrocytes in rats.

关 键 词：干细胞移植 放射疗法 脑损伤 星形细胞 

分 类 号：R739.91[医药卫生—肿瘤]