α-亚麻酸对戊酸雌二醇诱导的多囊卵巢综合征大鼠的影响  

The effect of alpha-Linolenic acid on EV-induced PCOS rats

作　　者：吴美丽 王德莹[2] 刘国 WU Meili;WANG Deying;LIU guo(Department of Gynecology,Qingdao Women and Children's Hospital,Qingdao 266011,Shandong,China;Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Harbin Medical University,Harbin 150080,Heilongjiang,China;Department of Gynecology,Affiliated Hospital of Binzhou Medical College,Binzhou 256603,Shandong,China)

机构地区：[1]青岛市妇女儿童医院妇科,山东青岛266011 [2]哈尔滨医科大学附属第二医院妇产科,黑龙江哈尔滨150080 [3]滨州医学院附属医院妇科,山东滨州256603

出　　处：《中南医学科学杂志》2020年第4期395-398,共4页Medical Science Journal of Central South China

摘　　要：探讨α-亚麻酸(ALA)对戊酸雌二醇(EV)诱导的多囊卵巢综合征大鼠的性激素水平及卵巢组织形态的影响。32只SD大鼠分为4组:对照组、模型组、ALA 50 mg/kg组和ALA 200 mg/kg组,每组各8只。采用HE染色方法观察大鼠卵巢组织形态,ELISA法检测血清中雌二醇(E2)、孕酮(P)、睾酮(T)和脱氢表雄酮(DHEA)含量。结果表明,与模型组大鼠比较,ALA处理组大鼠卵巢质量、E2、T水平均显著降低,P、卵巢组织中成熟卵泡数量及黄体数量均显著增多,且ALA均呈剂量依赖效应。ALA 200 mg/kg组大鼠卵巢组织中CYP11A1 mRNA含量低于模型组,差异具有统计学意义(P<0.05)。总体说明,ALA可明显改善戊酸雌二醇诱导的多囊卵巢综合征大鼠的卵巢组织形态,增加成熟卵泡及黄体数量,调节血清中性激素含量,降低卵巢组织中CYP11A1 mRNA表达。To explore the effect of alpha-Linolenic acid(ALA)on sex hoemone and morphology of ovarian tissue in EV-reduced PCOS.32 SD rats were divided into 4 groups:control group,model group,ALA 50mg/kg group and ALA 200mg/kg group,8 rats in each group.HE staining method was used to observe the morphology of rat ovary,and ELISA method was used to detect the changes of serum estradiol(E2),progesterone(P),testosterone(T)and dehydroepiandrosterone(DEHA).The result showed,compared to the model group,the ovarian mass,E2 and T levels in the ALA-treated group were significantly lower,the content of P,the number of mature follicles and corpus luteum in the ovarian tissue were significantly increased,and the effect of ALA displayed a dose-dependent manner.The content of CYP11A1 mRNA in ovarian tissue of ALA 200mg/kg group was lower than that of model group,the difference was statistically significant(P<0.05).Overall,ALA can significantly improve the ovarian tissue morphology of rats with polycystic ovary syndrome induced by estradiol valerate,increase the number of mature follicles and corpus luteum,regulate the content of serum sex hormones,and reduce the expression of CYP11A1 mRNA in ovarian tissue.

关 键 词：Α-亚麻酸 戊酸雌二醇 多囊卵巢综合征 性激素 大鼠 

分 类 号：R96[医药卫生—药理学]