精神分裂症断裂基因1基因多态性与帕利哌酮治疗精神分裂症疗效及微小RNA-134-5p关联性分析  

Analysis of efficacy and association between DISC1 gene polymorphism and palipiazone in the treatment of schizophrenia

作　　者：敬琳 杨昆[1] 姚静[1] 姜心梅 吴林林[1] JING Lin;YANG Kun;YAO Jing;JIANG Xinmei;WU Linlin(Department of Psychiatry,The Third Hospital of Manyang,Sichuan Mental Health Center,Manyang,Sichuan 621000,China)

机构地区：[1]绵阳市第三人民医院(四川省精神卫生中心)精神科,四川绵阳621000

出　　处：《安徽医药》2020年第8期1553-1557,共5页Anhui Medical and Pharmaceutical Journal

基　　金：四川省卫计委科研课题(18PJ315)。

摘　　要：目的观察精神分裂症断裂基因1(dierupted in schizophrenia 1,DISC1)基因多态性与帕利哌酮治疗精神分裂症疗效及微小RNA-134-5p(miR-134-5p)的关联性分析。方法选取2018年3月至2019年3月绵阳市第三人民医院精神分裂症病人109例为疾病组,采用帕利哌酮单药治疗,选取同期健康体检者109例为健康组,检测并比较两组DISC1基因单核苷酸多态性(SNPs)位点间差异。比较疾病组不同基因型病人帕利哌酮治疗前与治疗6个月后阳性和阴性症状量表(PANSS)、精神分裂症认知功能成套测验共识版(MCCB)评分以及血清miR-134-5p水平。结果 DISC1 SNPs位点比较中,疾病组rs821616位点与健康组差异有统计学意义(P<0.05)。疾病组不同基因型病人帕利哌酮治疗后PANSS评分均较治疗前降低,MCCB评分较治疗前升高(P<0.05)。rs821616基因型病人中TT型病人PANSS评分(46.3±7.1)分低于AA型病人(59.4±5.8)分,TT型病人MCCB评分(154.7±32.3)分高于AA型病人(131.3±32.1)分,AT型(0.32±0.07)和TT型(0.41±0.09)病人miR-134-5p水平高于AA型病人(0.30±0.11)。rs11122319基因型病人中GG型、AG型病人PANSS评分低于AA型病人,GG型病人MCCB评分和miR-134-5p水平高于AA型和AG型病人(P<0.05)。rs1417584基因型病人中GG型、AG型病人PANSS评分低于AA型病人,GG型病人MCCB评分高于AA型和AG型,GG型病人miR-134-5p水平高于AA型病人(P<0.05)。结论帕利哌酮可以改善精神分裂症病人症状,改善病人认知功能,提高miR-134-5p水平,不同DISC1基因型病人间治疗效果存在差异。Objective To investigate the association between polymorphism of schizophrenia 1(DISC1)gene and the therapeutic effect of paliperidone on patients with schizophrenia and miR-134-5 p.Methods One hundred and nine patients with schizophrenia in the Third People’s Hospital of Mianyang City from March 2018 to March 2019 were selected as the disease group and treated with paliperidone monotherapy.109 healthy subjects in the same period were enrolled in the study as control group.The different of single nucleotide polymorphism(SNPs)sites of DISC1 gene between the two groups were detected and compared.Positive and negative symptoms scale(PANSS),schizophrenia cognitive function test consensus test(MCCB)score,and serum miR-134-5 p level of patients with different genotypes of schizophrenia in disease group before and after 6 months of paliperidone treatment were compared.Results In the comparison of SNPs of DISC1,the difference of rs821616 locus between the disease group and the healthy group was statistically significant(P<0.05).The PANSS scores of patients with different genotypes in patients with schizophrenia decreased after treatment,and the MCCB scores were higher than those before treatment(P<0.05).Among the patients with rs821616 genotype,the PANSS score of patients with TT(46.3±7.1)was lower than that of AA(59.4±5.8)patients,and the MCCB score was higher than that of AA patients[(154.7±32.3)vs.(131.3±32.1)].The level of miR-134-5 p in patients with AT(0.32±0.07)and TT(0.41±0.09)was higher than that of AA patients(0.30±0.11).In patients with rs11122319 genotype,the PANSS scores of patients with GG and AG were lower than those of AA patients,the MCCB scores and miR-134-5 p levels of patients with GG were higher than those of AA and AG(all P<0.05).In patients with rs1417584 genotype,the PANSS scores of patients with GG and AG were lower than those of AA patients,the MCCB scores of patients with GG were higher than those of AA and AG,the level of miR-134-5 p in patients with GG was higher than that of AA pati

关 键 词：精神分裂症 多态性 单核苷酸 精神分裂症断裂基因1 微小RNA-134-5p 帕利哌酮 

分 类 号：R749.3[医药卫生—神经病学与精神病学]