RNAi下调CCT8抑制非小细胞肺癌A549细胞转移潜能  

RNAi down-regulates CCT8 to inhibit the metastatic potential of non-small cell lung cancer A549 cells

作　　者：朱迎伟[1] 褚旭 邱家勇 孙瑜霞[1] 毛毅敏[1] ZHU Yingwei;CHU Xu;QIU Jiayong;SUN Yuxia;MAO Yimin(Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital of Henan University of Science and Technology,Luoyang,Henan,China,471000)

机构地区：[1]河南科技大学第一附属医院呼吸与危重症医学科,河南洛阳471000

出　　处：《分子诊断与治疗杂志》2020年第7期897-900,909,共5页Journal of Molecular Diagnostics and Therapy

基　　金：河南省医学科技攻关计划(联合共建项目)(2018020285);洛阳市青年医学科技创新联合专项基金项目(1503008A-8)。

摘　　要：目的探讨RNAi下调CCT8对非小细胞肺癌A549细胞转移潜能的影响.方法A549细胞分为Control组、si-NC组、si-CCT8组、si-CCT8+IGF-1组,MTT方法测定细胞增殖,Transwell小室测定细胞侵袭和迁移,Western blot测定MMP-9、Vimentin、E-cadherin和p-Akt蛋白表达.结果与si-NC组相比,si-CCT8组细胞A值显著降低(P<0.05),细胞侵袭和迁移数量显著降低;与si-NC组相比,si-CCT8组MMP-9、Vimentin、p-Akt蛋白表达水平显著降低,E-cadherin蛋白表达水平显著升高(P<0.05).与si-CCT8组相比,si-CCT8+IGF-1组细胞A值、侵袭、迁移数量显著升高(P<0.05).结论RNAi下调CCT8抑制非小细胞肺癌A549细胞转移潜能,其作用机制与下调Akt信号激活程度有关.Objective To investigate the effect of RNAi down-regulation of CCT8 on the metastatic potential of non-small cell lung cancer A549 cells. Methods A549 cells were divided into Control group,si-NC group,si-CCT8 group,si-CCT8+IGF-1 group. MTT assay was used to measure cell proliferation,Transwell was used to measure cell invasion and Migration,Western blot was used to measure MMP-9,Vimentin,E-cadherin and p-Akt protein expression. Results Compared with si-NC group,the A value was significantly decreased,and the number of cell invasion and migration were significantly decreased in si-CCT8 group. Compared with si-NC group,the protein expression levels of MMP-9,Vimentin,p-Akt were significantly decreased,E-cadherin was significantly increased in si-CCT8 group(P<0.05). Compared with si-CCT8 group,the cell A value,the number of invasion,migration were significantly increased. Conclusion RNAi down-regulates CCT8 to inhibit the metastatic potential of non-small cell lung cancer A549 cells,and its mechanism is related to down-regulation of Akt signaling.

关 键 词：非小细胞肺癌 CCT8 Akt信号 

分 类 号：R734.2[医药卫生—肿瘤]