一株降解苯酚芦苇内生菌的分离与鉴定  

Isolation and Identification of a Phenol-degrading Endophyte from Phragmites Australis

作　　者：王清东 李玉玺 王乙羽 刘慧敏 刘俊华 张孝霖 WANG Qingdong(College of Biological and Environmental Engineering,Binzhou University,Binzhou 256600,China)

机构地区：[1]滨州学院生物与环境工程学院,山东滨州256600

出　　处：《安徽农学通报》2020年第14期40-41,84,共3页Anhui Agricultural Science Bulletin

基　　金：全国大学生创新训练计划(201710449012)。

摘　　要：为得到高效苯酚降解菌,以黄河三角洲盐碱地生芦苇(Phragmites australis)为材料,分离出1株能降解苯酚的芦苇内生菌L12,该菌能在以苯酚为唯一碳源的培养基上生长。采用4-氨基安替比林分光光度法测苯酚降解率,对该菌株的形态特征、理化性质、16S rDNA基因序列进行研究。结果表明:该菌株呈杆状,革兰氏染色呈阴性,初步鉴定为假单胞菌(Pseudmonas sp),能有效降解苯酚。In order to obtain a hyper efficient phenol-degrading bacterium strain, Phragmites australis in saline-alkali soil of the Yellow River Delta was used as the material to isolate a Phragmites australis endophytic bacteria L12,which was able to degrade phenol. The bacteria can survive on the medium with phenol as the sole carbon source.Phenol degradation rate was measured by 4-aminoantipyrine spectrophotometry. Further studies involving morphological characteristics, physicochemical properties and 16 S rDNA gene sequence showed that the strain was rodshaped and Gram-negative. It’s then identified as Pseudmonas sp. The results showed that a Pseudomonas endophyte was obtained from reed, which can degrade phenol effectively.

关 键 词：苯酚降解菌 芦苇 假单孢杆菌 理化性质 

分 类 号：Q93-331[生物学—微生物学]