竹叶黄酮提取物对HepG2细胞氧化应激的调节作用  

Bamboo Leaf Flavonoid C-glycosides Extracts Alleviate ROS Production Induced by Oleic Acid in HepG2 Cells

作　　者：张守丽[1] 陆梦琪 刘俊慧 俞玥 董燕军 ZHANG Shou-li;LU Meng-Qi;LIU Jun-Hui;YU Yue;DONG Yan-jun(Huzhou Vocational and Technological College,Huzhou 313000,China;School of Grain Science and Technology,Jiangsu University of Science and Technology,Zhenjiang 212004,China;Zhejiang Industrial Vocational and Technical College,Shaoxing 312000,China)

机构地区：[1]湖州职业技术学院,浙江湖州313000 [2]江苏科技大学粮食学院,江苏镇江212004 [3]浙江工业职业技术学院,浙江绍兴312000

出　　处：《中国果菜》2020年第6期65-70,76,共7页China Fruit & Vegetable

基　　金：浙江省教育厅科研项目(Y201840401)。

摘　　要：竹叶提取物具有良好的抗氧化、抗菌、消炎、抗心血管疾病等生物活性,已引起广泛关注。目前,关于竹叶黄酮提取物对高脂膳食引起的机体氧化应激干预作用的相关研究较少,鉴于此,本研究利用油酸诱导HepG2细胞建立了氧化应激细胞模型,探究竹叶提取物对氧化应激的干预作用。研究发现,相较于油酸处理24 h的损伤处理,竹叶提取物可以有效地降低诱导氧化应激细胞模型的甘油三酯(37%)、乳酸脱氢酶(34%)、丙二醛水平(49%),同时ROS水平降低了约32%,这表明竹叶提取物对于油酸引起的细胞损伤具有积极的干预作用。Bamboo leaf flavonoid extracts have attracted wide attention due to their good biological activities,such as antioxidation, antibacterial, anti-inflammatory and anti-cardiovascular diseases, and so on. However,there are rare studies of the effects of bamboo leaf flavonoids extracts on interfering oxidative stress induced by high-fat diet, and it is necessary to explore the related studies. In this study, HepG2 cells were induced by oleic acid to establish an oxidative stress damage cell model to present the protection effect of bamboo leaf flavonoid. It was concluded that bamboo leaf flavonoid extracts can effectively reduce the levels of triglycerides(37%), lactic acid dehydrogenase(34%), malondialdehyde(49%) and ROS(32%) to alleviate oxidative damage induced by oleic acid in HepG2 cells, indicating that bamboo leaf flavonoid extracts presented a positive intervention on high-fat-induced cell damage.

关 键 词：竹叶提取物 黄酮 油酸 氧化应激 活性氧 流式细胞仪 

分 类 号：TS201.2[轻工技术与工程—食品科学]