三七皂苷对脆性X综合征基因敲除小鼠水迷宫行为的影响  

Effect of Panax Notoginseng Saponins on Water Maze Behavior of Gene Knockout Mice with Fragile X Syndrome

作　　者：谢琼 蓝寿煌 陈劲松 钟成[2] 朱蔚云[3] 黄月玲[3] 戴丽军[3] 陈盛强[2] 丁松林 XIE Qiong;LAN Shouhuang;CHEN Jinsong;ZHONG Cheng;ZHU Weiyun;HUANG Yueling;DAI Lijun;CHEN Shengqiang;DING Songlin

机构地区：[1]乐昌市第二人民医院,广东乐昌512229 [2]广州医科大学附属第二医院,广东广州510260 [3]广州医科大学,广东广州510182

出　　处：《新中医》2020年第12期9-12,共4页New Chinese Medicine

基　　金：2018年度韶关市卫生计生科研项目(Y18008)。

摘　　要：目的:探究三七皂苷对脆性X综合征基因敲除小鼠水迷宫行为的影响。方法:将脆性X综合征基因敲除小鼠随机分成野生型(WT)组、基因敲除(KO)组、治疗组。治疗组采用30 mg/kg的三七皂苷对脆性X综合征基因敲除小鼠进行处理,用Morris水迷宫实验测试小鼠空间记忆能力,水迷宫实验共训练4 d,记录每天的潜伏期与游泳轨迹,第5天去除平台,记录小鼠停留各象限区域的时间百分比。结果:定向航行实验中,第1天和第2天,3组小鼠潜伏期变化差异无统计学意义(P>0.05);第3天和第4天,与WT组比较,KO组潜伏期增长(P<0.05);与KO组比较,治疗组潜伏期减小(P<0.05)。空间探索实验中,与WT组比较,KO组小鼠穿越平台次数明显减少(P<0.05),在第一象限停留时间减少;与KO组比较,治疗组小鼠穿越平台次数及第一象限停留时间均增加(P<0.05)。结论:KO小鼠存在认知功能障碍,30 mg/kg的三七皂苷对KO小鼠空间记忆能力有改善作用。Objective:To investigate the effect of panax notoginseng saponins on the water maze behavior of gene knockout mice with fragile X syndrome.Methods:Gene knockout mice with fragile X syndrome were randomly divided into the wild-type(WT)group,the knockout(KO)group and the treatment group.In the treatment group,30 mg/kg panax notoginseng saponins were used to treat gene knockout mice with fragile X syndrome.The Morris Water Maze Experiment was used to test the spatial memory ability of mice.The experiment lasted for four days,and the incubation period and swimming trajectory of each day were recorded.On Day 5,the platform was removed and the percentage of time the mice spent in each quadrant was recorded.Results:During the directional navigation experiment,there was no significant difference in the comparison of the incubation period between the three groups on Day 1 and Day 2(P>0.05).On Day 3 and day 4,the incubation period in the KO group was increased when compared with that in the WT group(P<0.05),and the incubation period in the treatment group was decreased when compared with that in the KO group(P<0.05).In the spatial search experiment,the number of crossing the platform in the KO group was decreased(P<0.05)and spent less time in the first quadrant when compared with that in the WT group;the number of crossing the platform in the treatment group was increased and spent more time in the first guadrant when compared with that in the KO group(P<0.05).Conclusion:KO mice had cognitive dysfunction,and 30 mg/kg of panax notoginseng saponins can improve spatial memory in KO mice.

关 键 词：脆性X综合征 三七皂苷 MORRIS水迷宫实验 动物实验 基因敲除小鼠 

分 类 号：R285.5[医药卫生—中药学]