高效液相色谱法测定新鲜烟叶中的多酚含量  

Determination of Polyphenols in Fresh Tobacco Leaves by High Performance Liquid Chromatography

作　　者：黄丽佳 梁梦洁 吴双凤 罗娜 季云瑞 王雪 郭亚东 邓亮 HUANG Lijia;LIANG Mengjie;WU Shuangfeng;LUO Na;JI Yunrui;WANG Xue;GUO Yadong;DENG Liang(College of Pharmaceutical Science&Yunnan Key Laboratory of Pharmacology for Nature Products,Kunming Medical University,Kunming,Yunnan,China 650500;Planing and Finance Division,Kunming Medical University,Kunming,Yunnan,China 650500)

机构地区：[1]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500 [2]昆明医科大学计划财务处,云南昆明650500

出　　处：《昆明学院学报》2020年第3期24-26,31,共4页Journal of Kunming University

基　　金：云南省科技厅科学研究基金资助项目(2018FB150);云南中烟工业有限责任公司资助项目(2018539200370184).

摘　　要：运用HPLC法测定新鲜烟叶中10种多酚的含量,色谱柱为CAPCELL PAK CR(4.6 mm I.D.×250 mm,5μm),柱温为30℃,二极管阵列检测器,检测波长340 nm.流动相流速0.8 m L/min,梯度洗脱.10种多酚在1~50μg/m L范围内具有良好的线性(r>0.999),加标回收率在90.0%~101.4%之间,RSD<5%.测定结果显示,新鲜烟叶中多酚含量分别为:绿原酸7106μg/g,云香苷5441μg/g,隐绿原酸5237μg/g,山奈酚苷1159μg/g,新绿原酸975μg/g,咖啡酸723μg/g,香豆素256μg/g,莨菪苷89μg/g,莨菪亭38μg/g,阿魏酸28μg/g.The contents of ten polyphenols in fresh tobacco leaves were determined by high performance liquid chromatography(HPLC)with chromatographic column CAPCELL PAK CR(4.6 mm I.D.×250 mm,5μm);column temperature 30℃;DAD detector with detection wavelength 340 nm and mobile phase flow rate 0.8 mL/min for gradient elution.Ten polyphenols have good linearity(r>0.999)in the range of 1—50μg/mL,and the spiked recoveries were between 90.0%—101.4%,RSD<5%.The measurement results showed that the contents of the polyphenols in fresh tobacco leaves were(μg/g):chlorogenic acid 7106,rutin 5441,cryptogenic acid 5237,kaempferol 1159,neochlorogenic acid 975,caffeic acid 723,coumarin 256,guanidine 89,scopletin 38 and ferulaic acid 28.

关 键 词：新鲜烟叶 高效液相色谱法 多酚 测定 

分 类 号：S527[农业科学—作物学]