自噬相关基因LC3和ATG5在胃癌中的表达及临床意义  

Expression and clinical significance of autophagy-relatedgenes LC3 and ATG5 in gastric cancer

作　　者：许祥云 陈书昌 孔德九 侯欣欣 陈强[1] 陈静[1] XU Xiangyun;CHEN Shuchang;KONG Dejiu;HOU Xinxin;CHEN Qiang;CHEN Jing(The First Affiliated Hospital,College of Clinical Medicine of Henan University of Science and Technology,Luoyang 471003,China)

机构地区：[1]河南科技大学临床医学院,河南科技大学第一附属医院,河南洛阳471003

出　　处：《实用医学杂志》2020年第14期1939-1945,共7页The Journal of Practical Medicine

基　　金：河南省医学科技攻关计划项目(编号:201504036)。

摘　　要：目的探讨自噬相关基因微管相关蛋白1轻链3(microtubule-associated protein 1 light chain 3,LC3)和自噬相关基因-5(autophagy related gene-5,ATG5)在胃癌细胞发生发展不同阶段的表达情况并分析其临床意义。方法选取胃黏膜正常细胞、胃黏膜不典型增生细胞、胃癌细胞共58例,分别记为正常组(17例)、不典型组(19例)、胃癌组(22例),采用qRT-PCR、Western Blot、免疫组织化学法检测每组中自噬相关基因LC3和ATG5的mRNA及蛋白表达水平,应用SPSS 23.0软件分析正常组、不典型组、胃癌组3组间LC3和ATG5的mRNA及蛋白表达情况,并分析LC3和ATG5蛋白表达的相关性以及LC3和ATG5蛋白表达与胃癌患者临床病理学参数的关系。结果正常组、不典型组、胃癌组中LC3和ATG5 mRNA及蛋白表达水平逐渐升高,各组间差异均有统计学意义(P<0.05)。LC3蛋白表达情况与性别、年龄、浸润深度、分化程度及淋巴结状态均不存在相关性。ATG5蛋白表达情况与浸润深度、分化程度及淋巴结状态均有相关性。LC3和ATG5蛋白在3组中均呈正相关(P<0.05)。结论细胞自噬可促进胃癌的发生发展,其机制可能与LC3、ATG5过度表达有关。LC3、ATG5在促进胃癌的发生发展中起相互协同的作用。Objective To investigate the expression of autophagy-related genes LC3 and ATG5 in differentstages of gastric cancer and analyze their clinical significance.Methods58 cases of normal gastric mucosa,dys-plasia gastric mucosa and gastric cancer were selected and recorded as normal group(17 cases),atypical group(19 cases)and gastric cancer group(22 cases)respectively. The m RNA and protein expression of LC3 and ATG5 were detected by q RT-PCR,Western Blot,immunohistochemistry,and SPSS 23.0 software was used to analyzethe m RNA and protein expression level of LC3 and ATG5 in normal group,atypical group and gastric cancer group,to analyze the correlation between LC3 and ATG5 protein expression,and to analyze the relationship between theexpression of LC3 and ATG5 and the clinicopathological parameters of gastric cancer.ResultsThe m RNA andprotein expression levels of LC3 and ATG5 in the normal group,the atypical group and the gastric cancer groupincreased gradually,and there were significant differences among groups(P<0.05). There was no correlationbetween the expression of LC3 protein and sex,age,invasion depth,differentiation degree and lymph node status.The expression of ATG5 protein was significantly correlated with infiltration depth,differentiation degree and lymphnode status. There was a positive correlation between LC3 and ATG5(P<0.05).ConclusionAutophagy can pro-mote the development of gastric cancer,and its mechanism may be related to the overexpression of LC3 and ATG5.LC3 and ATG5 play a synergistic role in promoting the occurrence and development of gastric cancer.

关 键 词：胃癌 自噬 微管相关蛋白1轻链3(LC3) 自噬相关基因-5(ATG5) 

分 类 号：R735.2[医药卫生—肿瘤]