伊维菌素对宫颈癌Hela细胞增殖和凋亡的影响  

Effect of ivermectin on proliferation and apoptosis of cervical cancer Hela cells

作　　者：金亚玲 王祥珍[1] 刘厚聪 孟军 JIN Ya-ling;WANG Xiang-zhen;LIU Hou-cong;MENG Jun(Department of Gynecology and Obstetrics,Nanshan Maternal and Child Health Hospital,Shenzhen 518067,China;Central Laboratory,Shenzhen Union Hospital,Huazhong University of Science and Technology,Shenzhen 518067,China;Department of Gynecology and Obstetrics,Shenzhen Union Hospital,Huazhong University of Science and Technology,Shenzhen 518067,China)

机构地区：[1]广东省深圳市南山区妇幼保健院妇产科,广东深圳518067 [2]华中科技大学协和深圳医院中心实验室,广东深圳518000 [3]华中科技大学协和深圳医院妇产科,广东深圳518000

出　　处：《广东医科大学学报》2020年第4期391-394,共4页Journal of Guangdong Medical University

基　　金：广东省医学科学技术研究基金项目(No.C2017034)。

摘　　要：目的探讨伊维菌素对人宫颈癌Hela细胞增殖和凋亡的影响。方法0.1、1、3μmol/L伊维菌素处理Hela细胞,MTT、Edu法检测细胞活性,流式细胞术检测细胞凋亡,蛋白印迹检测p-JNK、JNK、NF-κB p65、Bcl-2、Caspase-3、Caspase-9表达。结果伊维菌素呈浓度依赖性抑制Hela细胞增殖、诱导凋亡(P<0.01),上调p-JNK、Caspase-3、Caspase-9表达,下调NF-κB p65、Bcl-2表达。结论伊维菌素可能通过上调JNK信号通路诱导Hela细胞凋亡,下调NF-κB信号通路抑制Hela细胞增殖。Objective To investigate the effect of ivermectin on proliferation and apoptosis of cervical cancer Hela cells.Methods After treated with 0.1,1,3μmol/L ivermectin,viability,apoptosis,and expression of p-JNK,JNK,NF-κB p65,Bcl-2,Caspase-3 and Caspase-9 in Hela cells were determined by MTT,Edu,flow cytometry,Western blot,respectively.Results Ivermectin inhibited proliferation,induced apoptosis(P<0.01),up-regulated expression of p-JNK,Caspase-3 and Caspase-9,and down-regulated expression of NF-κB p65 and Bcl-2 in Hela cells in a dose-dependent manner.Conclusion Ivermectin could induce apoptosis via up-regulation of JNK pathway and inhibit proliferation through down-regulation of NF-κB signaling in Hela cells.

关 键 词：伊维菌素 HELA细胞 增殖 凋亡 

分 类 号：R739[医药卫生—肿瘤]