甘肃省安定区马铃薯黑痣病病菌菌丝融合群鉴定及其越冬能力初探  

Identification and overwintering ability test of Rhizoctonia solani anastomosis groups in potato in the Anding district of Gansu province

作　　者：王银钰 李青 杨成德 张俊莲[2] Wang Yinyu;Li Qing;Yang Chengde;Zhang Junlian(College of Plant Protection,Gansu Agricultural Universi ty/Laboratory of Crop Pest and Disease Biological Control Project in Gansu Province,Lanzhou Gansu 730070,China;Gansu Key Laboratory of Crop Genetic Improvement and Germplasm Innovation,Lanzhou Gansu 730070,China)

机构地区：[1]甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室,甘肃兰州730070 [2]甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070

出　　处：《中国植保导刊》2020年第6期11-16,共6页China Plant Protection

基　　金：国家马铃薯产业技术体系项目(CARS-10-P18)。

摘　　要：从甘肃省安定区采集了4个品种的马铃薯黑痣病薯块共18个样本,采用常规组织分离法分离纯化得到98株具立枯丝核菌典型特征的菌株,经DAPI染液观察其核相,结合载玻片配对法测定其菌丝融合群。结果表明,98株立枯丝核菌均为多核,介于3~25个之间。其中,AG3融合群20株(占总菌株数20.4%),AG5融合群60株(占总菌株数61.2%),AG9融合群8株(占总菌株数8.2%),10株与标准菌株均不融合(占总菌株数10.2%)。4个品种中不同菌丝融合群分离频率有差异。AG5融合群的分离频率均最高。其中,在庄薯3号的分离频率达65.5%,为甘肃省安定区马铃薯黑痣病优势菌丝融合群,且其越冬能力强于其他菌丝融合群。A total of 18 potato tuber samples with black scurf disease were collected from four cultivars in the Anding district of Gansu province,and 98 strains with typical characteristics of Rhizoctonia solani were isolated and purified by conventional tissue isolate method.The nuclear phase was observed by the DAPI staining,and the anastomosis group was determined by the paired slide method.The results showed that the 98 isolates of Rhizoctonia solani were multinucleated,and the nuclear numbers were ranging from 3 to 25.Among them,20,60,and 8 isolates were in the AG3,AG5,and AG9 anastomosis group,accounting for 20.4%,61.2%,and 8.2%of total isolates,and 10 isolates did not fuse with standard isolates,accounting for 10.2%of the total.The isolation frequencies of different dominant anastomosis groups were different in 4 potato cultivars.The AG5 anastomosis group had the highest isolation frequency,especially in Zhuangshu No.3,which reached 65.5%.It is the dominant anastomosis group of Rhizoctonia solani in the Anding District,and its overwintering ability is stronger than other anastomosis groups.

关 键 词：马铃薯 黑痣病病菌 核相观察 菌丝融合群 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]