逐水饮治疗恶性胸腔积液大鼠的效果及对其胸膜组织TGF-β1、AQP-1表达的影响  

Effect of Zhushui Yin on rats with malignant pleural effusion and its effect on expression of TGF-β1 and AQP-1 in rat pleural tissues

作　　者：刘晓芳 王晓婷 赵楠 LIU Xiaofang;WANG Xiaoting;ZHAO Nan(College of Acupuncture and Massage,Heilongjiang University of Chinese Medicine,Heilongjiang Province,Harbin 150040,China;Department of Oncology,Harbin Chinese Medicine Hospital,Heilongjiang Province,Harbin 150900,China)

机构地区：[1]黑龙江中医药大学针灸推拿学院,黑龙江哈尔滨150040 [2]黑龙江省哈尔滨市中医院肿瘤科,黑龙江哈尔滨150900

出　　处：《中国医药导报》2020年第21期17-21,共5页China Medical Herald

基　　金：黑龙江省中医药科研项目(ZHY16-022)。

摘　　要：目的研究逐水饮对大鼠恶性胸腔积液的治疗作用及对大鼠胸膜组织转化生长因子-β1(TGF-β1)和水通道蛋白-1(AQP-1)表达的影响。方法建立恶性胸腔积液大鼠模型,将建模成功的大鼠按随机数字表法分为5组,分别为模型组(MC)、阳性对照组(PC)、逐水饮高剂量组(HD)、逐水饮中剂量组(MD)、逐水饮低剂量组(LD),每组10只。另取10只正常大鼠记为正常组(NC)。建模后第1天,PC腹腔注射2 mg/kg顺铂注射液,1次/2 d;HD、MD和LD分别灌胃66.0、33.0、16.5 g/kg的逐水饮药液,1次/d;NC和MC灌胃等体积的生理盐水,1次/d,共7 d。比较各组胸腔积液量和癌细胞含量;苏木精-伊红染色观察胸膜组织病变;比较各组TGF-β1和AQP-1表达。结果MC、PC、HD、MD和LD第4、8天大鼠胸腔积液量、癌细胞数均高于第1天,第8天均高于第4天;第1天MC大鼠胸腔积液量高于NC,第4、8天MC大鼠胸腔积液量、癌细胞数均高于NC,PC、HD、MD、LD均低于MC,HD低于PC,LD高于PC,差异均有统计学意义(均P<0.05)。NC大鼠胸膜组织结构正常。MC大鼠胸膜增厚严重,癌细胞、淋巴细胞浸润严重。HD、MD、LD胸膜层厚减轻,癌细胞浸润较少。与NC比较,MC TGF-β1和AQP-1表达水平升高(P<0.05);与MC比较PC、HD、MD和LD TGF-β1和AQP-1表达水平均降低(P<0.05);与PC比较,HD TGF-β1和AQP-1表达水平降低,LD TGF-β1和AQP-1表达水平升高,差异均有统计学意义(均P<0.05)。结论逐水饮对大鼠恶性胸腔积液疗效良好,可能与下调TGF-β1、AQP-1表达有关。Objective To investigate the therapeutic effect of Zhushui Yin on malignant pleural effusion in rats and its effect on expression of transforming growth factor-β1(TGF-β1)and aquaporin-1(AQP-1)in rat pleural tissues.Methods The rat model of malignant pleural effusion was established,and the successfully modeled rats were divided into five groups according to the random number table method,namely,the model group(MC),the positive control group(PC),the high-dose group(HD),the middle-dose group(MD),and the low-dose group(LD),with ten rats in each group.Another ten normal rats were recorded as normal group(NC).On the first day after modeling,PC was intraperitoneally injected with 2 mg/kg Cisplatin Injection once every two days.HD,MD and LD were respectively given 66.0,33.0 g/kg and 16.5 g/kg of Zhushui Yin Liquid once a day.NC and MC were given the same volume of normal saline,once a day,for seven days in total.The amount of pleural effusion and the content of cancer cells in each group were compared.Pleural lesions were observed by hematoxylin-Eosin staining.The TGF-β1 and AQP-1 expression were compared in each group.Results The amount of pleural effusion and the number of cancer cells of MC,PC,HD,MD and LD on the fourth day and the eighth day were all higher than those on the first day,and the eighth day was higher than the fourth day.On the first day,the pleural effusion amount of MC rats was higher than NC,on the fourth day and the eighth day,the pleural effusion amount and cancer cell number of MC rats were higher than those of NC,while PC,HD,MD and LD were lower than MC,HD was lower than PC,LD was higher than PC,and the differences were statistically significant(all P<0.05).The pleural structure of NC rats was normal.The pleural thickening and infiltration of cancer cells and lymphocytes were serious in MC rats. The pleural thickness in HD, MD, LD was reduced and the infiltration of cancer cells was less. Compared with NC, the expressions of TGF-β1 and AQP-1 in MC were increased (P < 0.05). Compared with MC,

关 键 词：逐水饮 恶性胸腔积液 转化生长因子-Β1 水通道蛋白-1 

分 类 号：R737.11[医药卫生—肿瘤]