ASPM基因在肾透明细胞癌中的表达及其诊断预后的价值  

Expression and prognostic value of ASPM in renal clear cell carcinoma

作　　者：陈悦康[1] 王伟[1] 卢启海[1] 姚远[1] 杨剑文[1] CHEN Yuekang;WANG Wei;LU Qihai;YAO Yuan;YANG Jianwen(Department of Urology,Liuzhou People's Hospital,Liuzhou,Guangxi,545001)

机构地区：[1]柳州市人民医院泌尿外科,广西柳州545001

出　　处：《临床泌尿外科杂志》2020年第5期398-402,共5页Journal of Clinical Urology

基　　金：广西卫健委自筹经费科研课题(No:Z20190023)。

摘　　要：目的:探讨人类异常纺锤体样小头畸形相关蛋白(abnormal spindle-like microcephaly-associated protein,ASPM)在肾透明细胞癌中的表达情况以及其诊断预后的价值。方法:利用癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库的RNA-Seq数据和临床数据,分析ASPM基因与肾透明细胞癌患者临床病理特征以及生存预后的关系。结果:总体534例肾透明细胞癌样本的癌和癌旁组织中的ASPM的表达水平分别为6.62±1.34和3.59±1.41,差异有统计学意义(P<0.001)。72例配对的肾透明细胞癌和癌旁组织中的ASPM表达水平分别为6.89±1.14和3.59±1.41,差异有统计学意义(P<0.001)。ROC曲线分析结果表明,ASPM基因可作为一个灵敏度和特异度较高的肾透明细胞癌诊断指标(敏感性为94.2%,特异性为90.3%,AUC=0.935)。ASPM基因表达与肾透明细胞癌患者临床病理特征的关系显示,ASPM表达与性别、肿瘤分级、T分期、M分期、临床分期均有显著性差异(P<0.001)。生存分析发现总体534例样本中ASPM低表达者和高表达者的总体生存时间分别为(1421.85±984.72)d和(1279.89±976.68)d,比较差异有统计学意义(Log-rank=14.69,P<0.001)。结论:ASPM在肾透明细胞癌中高表达,且临床特征密切相关,其高表达对判断肾透明细胞癌的生存预后及其诊断具有参考价值。Objective:To investigate the expression of abnormal spindle-like microcephaly-associated protein(ASPM)in renal clear cell carcinoma and its prognostic value.Method:The RNA-Seq data and clinical data of the Cancer Genome Atlas(TCGA)database were used to analyze the relationship between ASPM gene and clinicopathological features and survival prognosis in patients with renal clear cell carcinoma.Result:The expression levels of ASPM in cancer and paracancerous tissues of 534renal clear cell carcinoma samples were 6.62±1.34and 3.59±1.41,respectively,and the difference was statistically significant(P<0.001).The expression levels of ASPM in 72matched renal clear cell carcinoma and adjacent tissues were 6.89±1.14and 3.59±1.41,respectively,and the difference was statistically significant(P<0.001).The ROC curve analysis showed that the ASPM gene can be used as a diagnostic index for renal clear cell carcinoma with high sensitivity and specificity(Sensitivity:94.2%,Specificity:90.3%,AUC=0.935).The relationship between ASPM gene expression and clinicopathological features of patients with renal clear cell carcinoma showed that ASPM expression was significantly different from gender,tumor grade,T stage,M stage,and clinical stage.Survival analysis found that the overall survival time of ASPM low expression and high expression in the overall 534samples were(1421.85±984.72)and(1279.89±976.68)days,respectively,and the difference was statistically significant(Log-rank=14.69,P<0.001).Conclusion:ASPM is highly expressed in renal clear cell carcinoma and is closely related to clinical features.Its high expression has reference value for judging the survival prognosis and diagnosis of renal clear cell carcinoma.

关 键 词：ASPM基因 肾透明细胞癌 TCGA 生物信息学 

分 类 号：R737.11[医药卫生—肿瘤]